Detailed information of TA system TA070657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 404021..404659
• Replicon: chromosome
• Accession: NZ_CP018839
• Organism: Thauera chlorobenzoica strain 3CB1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1H5W8T7
• Locus tag: Tchl_RS01910
• Protein ID: WP_075146901.1
• Coordinates: 404258..404659 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1H5W8M0
• Locus tag: Tchl_RS01905
• Protein ID: WP_075146900.1
• Coordinates: 404021..404251 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Tchl_RS01890	400883..401206	+	324	WP_075146897.1	flagellar hook-basal body complex protein FliE	-
Tchl_RS01895	401291..401653	-	363	WP_075146898.1	flagellar protein FlaG	-
Tchl_RS01900	401714..403708	-	1995	WP_075146899.1	methyl-accepting chemotaxis protein	-
Tchl_RS01905	404021..404251	+	231	WP_075146900.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
Tchl_RS01910	404258..404659	+	402	WP_075146901.1	PIN domain-containing protein	Toxin
Tchl_RS01915	404945..405331	-	387	WP_075146902.1	type II toxin-antitoxin system VapC family toxin	-
Tchl_RS01920	405443..405754	+	312	WP_075146703.1	IS66 family insertion sequence hypothetical protein	-
Tchl_RS01925	405754..406113	+	360	WP_083945075.1	IS66 family insertion sequence element accessory protein TnpB	-
Tchl_RS01930	406143..407759	+	1617	WP_075146702.1	IS66 family transposase	-
Tchl_RS01935	407805..408059	-	255	WP_198158978.1	Arc family DNA-binding protein	-
Tchl_RS01940	408337..408624	-	288	WP_075149528.1	HigA family addiction module antidote protein	-
Tchl_RS17895	408637..408711	-	75	Protein_385	type II toxin-antitoxin system RelE/ParE family toxin	-
Tchl_RS01950	408943..409368	-	426	WP_198158979.1	putative toxin-antitoxin system toxin component, PIN family	-
Tchl_RS01955	409365..409589	-	225	WP_075146905.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-40)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14134.31 Da
• Isoelectric Point: 6.7267

>T70657 WP_075146901.1 NZ_CP018839:404258-404659 [Thauera chlorobenzoica]
MIHLLDTNIFIAALKAHPTVRARLESLPASAVALSPIVLGELQTGVEKSEQVERNRARLEQVVAGLAVPGVDAATSAHYA
RIRAALERQGTPIGGNDLWIAAQALALGAVLVTDNVKEFSRVPGLVVENWLAQ

Nucleotide

• Length: 402 bp

>T70657 NZ_CP018839:404258-404659 [Thauera chlorobenzoica]
ATGATCCACCTGCTCGATACCAACATCTTCATCGCCGCGCTGAAGGCCCACCCAACGGTTCGCGCGCGGCTCGAGTCCTT
GCCGGCCTCCGCTGTCGCTCTTTCGCCCATCGTGCTGGGCGAACTCCAGACCGGTGTGGAGAAGAGCGAACAGGTCGAGC
GTAACCGTGCACGGCTGGAGCAGGTGGTTGCGGGGCTTGCGGTGCCGGGGGTGGATGCCGCGACGAGCGCCCACTATGCA
CGGATCCGTGCCGCGCTCGAGCGGCAAGGTACGCCGATCGGTGGCAATGATCTCTGGATTGCGGCACAGGCACTTGCACT
GGGTGCAGTGCTGGTCACCGATAACGTGAAGGAGTTCAGCCGCGTTCCGGGCCTGGTGGTGGAGAACTGGCTGGCTCAGT
GA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8005.08 Da
• Isoelectric Point: 4.4472

>AT70657 WP_075146900.1 NZ_CP018839:404021-404251 [Thauera chlorobenzoica]
MRTVAVVEAKSQFSALLAAVESGEEVAITRRGRVVARLVPDTPRSAADVFRPCWQDGGLDLAAPADALAEPVAGWD

Nucleotide

• Length: 231 bp

>AT70657 NZ_CP018839:404021-404251 [Thauera chlorobenzoica]
ATGCGTACCGTTGCGGTTGTCGAGGCCAAGAGCCAGTTTTCTGCGCTGTTGGCGGCAGTGGAGAGTGGCGAGGAGGTGGC
GATCACCCGGCGTGGGCGAGTCGTCGCTCGTCTGGTGCCGGATACGCCGCGTTCGGCGGCGGATGTATTTCGGCCCTGCT
GGCAGGATGGCGGGCTCGATCTGGCGGCGCCGGCCGATGCGCTGGCCGAGCCGGTCGCCGGGTGGGATTGA

Structures

Toxin

• AlphaFold DB: A0A1H5W8T7

Antitoxin

• AlphaFold DB: A0A1H5W8M0