Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 540931..541502 Replicon chromosome
Accession NZ_CP018828
Organism Enterococcus faecium strain ISMMS_VRE_12

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag AL026_RS04715 Protein ID WP_002286801.1
Coordinates 541161..541502 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag AL026_RS04710 Protein ID WP_002323011.1
Coordinates 540931..541161 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL026_RS04675 536064..537395 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
AL026_RS04680 537417..538043 + 627 WP_002286816.1 cysteine hydrolase -
AL026_RS04685 538226..538807 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
AL026_RS04690 539539..539886 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
AL026_RS04695 539862..540101 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
AL026_RS04700 540306..540644 - 339 WP_002286804.1 hypothetical protein -
AL026_RS04710 540931..541161 + 231 WP_002323011.1 hypothetical protein Antitoxin
AL026_RS04715 541161..541502 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AL026_RS04720 542535..543722 - 1188 WP_002296840.1 IS256-like element IS16 family transposase -
AL026_RS04735 544516..544710 + 195 WP_002297028.1 hypothetical protein -
AL026_RS04740 544868..545150 + 283 Protein_511 transposase -
AL026_RS04745 545394..546224 - 831 WP_002286789.1 manganese catalase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 542535..543722 1187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T70644 WP_002286801.1 NZ_CP018828:541161-541502 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T70644 NZ_CP018828:541161-541502 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT70644 WP_002323011.1 NZ_CP018828:540931-541161 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT70644 NZ_CP018828:540931-541161 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References