Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1397..2004 Replicon plasmid p1_ISMMS_VRE12
Accession NZ_CP018827
Organism Enterococcus faecium strain ISMMS_VRE_12

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag AL026_RS00320 Protein ID WP_002321032.1
Coordinates 1654..2004 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag AL026_RS00315 Protein ID WP_002287514.1
Coordinates 1397..1660 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL026_RS00310 14..1159 + 1146 WP_002305757.1 IS200/IS605 family element transposase accessory protein TnpB -
AL026_RS00315 1397..1660 + 264 WP_002287514.1 hypothetical protein Antitoxin
AL026_RS00320 1654..2004 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AL026_RS00325 2028..2642 - 615 WP_002318230.1 recombinase family protein -
AL026_RS00330 3092..4417 + 1326 WP_002348911.1 Y-family DNA polymerase -
AL026_RS00335 4410..4760 + 351 WP_002303114.1 hypothetical protein -
AL026_RS00340 4757..4936 + 180 WP_002322386.1 hypothetical protein -
AL026_RS00345 4948..5241 + 294 WP_002322251.1 hypothetical protein -
AL026_RS00350 5467..6285 + 819 WP_002305212.1 ParA family protein -
AL026_RS00355 6304..6711 + 408 WP_002305774.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..259837 259837
- flank IS/Tn - - 14..1159 1145


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T70641 WP_002321032.1 NZ_CP018827:1654-2004 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T70641 NZ_CP018827:1654-2004 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT70641 WP_002287514.1 NZ_CP018827:1397-1660 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT70641 NZ_CP018827:1397-1660 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References