Detailed information of TA system TA070628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 73329..73972
• Replicon: plasmid unitig_3
• Accession: NZ_CP018819
• Organism: Klebsiella pneumoniae strain AR_0049

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: AM428_RS30820
• Protein ID: WP_001044770.1
• Coordinates: 73329..73745 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: AM428_RS30825
• Protein ID: WP_001261282.1
• Coordinates: 73742..73972 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM428_RS30780	69181..69885	+	705	WP_020324562.1	IS6-like element IS26 family transposase	-
AM428_RS30790	70191..70748	+	558	WP_001217881.1	recombinase family protein	-
AM428_RS30795	70982..71536	+	555	WP_063840280.1	AAC(6')-Ib family aminoglycoside 6'-N-acetyltransferase	-
AM428_RS30800	71606..71797	+	192	Protein_84	nucleotidyltransferase domain-containing protein	-
AM428_RS30805	71814..72511	-	698	Protein_85	IS1 family transposase	-
AM428_RS30815	72545..73255	-	711	WP_004152334.1	ATP-binding protein	-
AM428_RS30820	73329..73745	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM428_RS30825	73742..73972	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM428_RS30830	73929..74156	+	228	Protein_89	hypothetical protein	-
AM428_RS30840	74483..74935	-	453	WP_004201034.1	hypothetical protein	-
AM428_RS30855	75743..76093	+	351	WP_000493378.1	hypothetical protein	-
AM428_RS30860	76144..76887	+	744	WP_000129823.1	hypothetical protein	-
AM428_RS30865	76884..77660	+	777	WP_000015958.1	site-specific integrase	-
AM428_RS30870	77718..77975	-	258	WP_000764642.1	hypothetical protein	-
AM428_RS33275	78104..78208	-	105	WP_032409716.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / blaSHV-187 / qnrB9 / dfrA14 / aac(6')-Ib	-	1..85161	85161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T70628 WP_001044770.1 NZ_CP018819:c73745-73329 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T70628 NZ_CP018819:c73745-73329 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT70628 WP_001261282.1 NZ_CP018819:c73972-73742 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT70628 NZ_CP018819:c73972-73742 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3