Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 48338..49074 Replicon plasmid unitig_2
Accession NZ_CP018818
Organism Klebsiella pneumoniae strain AR_0049

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM428_RS29860 Protein ID WP_003026803.1
Coordinates 48592..49074 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM428_RS29855 Protein ID WP_003026799.1
Coordinates 48338..48604 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM428_RS29815 43429..43776 + 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
AM428_RS29820 43825..45363 + 1539 WP_004201219.1 IS66-like element ISKpn24 family transposase -
AM428_RS29835 46262..46570 - 309 WP_017896554.1 hypothetical protein -
AM428_RS29845 47064..47612 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
AM428_RS29850 47659..48093 + 435 WP_001567367.1 cell envelope integrity protein TolA -
AM428_RS29855 48338..48604 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM428_RS29860 48592..49074 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM428_RS29865 49275..50678 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AM428_RS29870 50707..51339 - 633 WP_001567369.1 hypothetical protein -
AM428_RS29875 51553..51759 + 207 Protein_53 helix-turn-helix domain-containing protein -
AM428_RS29880 51957..53720 + 1764 WP_004200842.1 DUF262 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / mph(A) - 1..117755 117755
- inside IScluster/Tn - - 43028..50678 7650


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T70627 WP_003026803.1 NZ_CP018818:48592-49074 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T70627 NZ_CP018818:48592-49074 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT70627 WP_003026799.1 NZ_CP018818:48338-48604 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT70627 NZ_CP018818:48338-48604 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References