Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 43726..44369 Replicon plasmid tig00000003
Accession NZ_CP018815
Organism Enterobacter cloacae complex sp. AR_0002

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AM329_RS24510 Protein ID WP_001044770.1
Coordinates 43953..44369 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AM329_RS24505 Protein ID WP_001261282.1
Coordinates 43726..43956 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM329_RS24475 (AM329_23695) 39443..40198 - 756 WP_012539983.1 replication initiation protein RepE -
AM329_RS25965 40829..40942 + 114 WP_032072094.1 hypothetical protein -
AM329_RS24485 41071..41328 + 258 WP_032408936.1 hypothetical protein -
AM329_RS24490 (AM329_23700) 41386..42171 - 786 WP_022644886.1 site-specific integrase -
AM329_RS24495 (AM329_23705) 42174..42812 - 639 WP_022644887.1 hypothetical protein -
AM329_RS25830 42861..43181 - 321 WP_032072060.1 hypothetical protein -
AM329_RS24500 43309..43769 - 461 Protein_62 hypothetical protein -
AM329_RS24505 (AM329_23710) 43726..43956 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
AM329_RS24510 (AM329_23715) 43953..44369 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AM329_RS24515 (AM329_23720) 44443..44661 + 219 Protein_65 AAA family ATPase -
AM329_RS24520 (AM329_23725) 44726..45430 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
AM329_RS24525 45483..46250 - 768 Protein_67 class 1 integron integrase IntI1 -
AM329_RS24535 (AM329_23735) 46406..46879 + 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
AM329_RS26190 47100..47216 + 117 Protein_69 plasmid mobilization relaxosome protein MobC -
AM329_RS26195 47326..47391 + 66 Protein_70 EAL domain-containing protein -
AM329_RS24555 (AM329_23745) 47509..48273 + 765 WP_001389365.1 IS6-like element IS6100 family transposase -
AM329_RS25970 48413..48478 - 66 Protein_72 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaKPC-3 - 1..139942 139942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T70607 WP_001044770.1 NZ_CP018815:43953-44369 [Enterobacter cloacae complex sp. AR_0002]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T70607 NZ_CP018815:43953-44369 [Enterobacter cloacae complex sp. AR_0002]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT70607 WP_001261282.1 NZ_CP018815:43726-43956 [Enterobacter cloacae complex sp. AR_0002]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT70607 NZ_CP018815:43726-43956 [Enterobacter cloacae complex sp. AR_0002]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References