Detailed information of TA system TA070604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3862547..3863154
• Replicon: chromosome
• Accession: NZ_CP018814
• Organism: Enterobacter cloacae complex sp. AR_0002

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4Q4AAS0
• Locus tag: AM329_RS19085
• Protein ID: WP_057979982.1
• Coordinates: 3862547..3862732 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM329_RS19090
• Protein ID: WP_023315016.1
• Coordinates: 3862747..3863154 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM329_RS19070 (AM329_18480)	3857866..3859704	+	1839	WP_023323871.1	selenocysteine-specific translation elongation factor	-
AM329_RS19075 (AM329_18485)	3859708..3860592	-	885	WP_003860164.1	ROK family protein	-
AM329_RS19080 (AM329_18490)	3860759..3862297	+	1539	WP_006808612.1	aldehyde dehydrogenase AldB	-
AM329_RS19085	3862547..3862732	+	186	WP_057979982.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM329_RS19090 (AM329_18495)	3862747..3863154	+	408	WP_023315016.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM329_RS19095 (AM329_18500)	3863158..3864234	-	1077	WP_023323870.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
AM329_RS19100 (AM329_18505)	3864695..3865150	+	456	WP_023323869.1	4Fe-4S dicluster domain-containing protein	-
AM329_RS19105 (AM329_18510)	3865158..3866411	-	1254	WP_015572749.1	valine--pyruvate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6852.08 Da
• Isoelectric Point: 11.5191

>T70604 WP_057979982.1 NZ_CP018814:3862547-3862732 [Enterobacter cloacae complex sp. AR_0002]
VKSADIITVLVSHGWKCVRTKGSHHQFRHPVQKGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Nucleotide

• Length: 186 bp

>T70604 NZ_CP018814:3862547-3862732 [Enterobacter cloacae complex sp. AR_0002]
GTGAAAAGTGCGGATATCATTACTGTTCTGGTTAGCCATGGCTGGAAATGTGTAAGAACGAAGGGAAGCCATCATCAGTT
TCGCCATCCAGTGCAAAAAGGGTTGGTTACCGTCCCACATCCTAAAAAGGACATTAAACCAGGCACTCTCGCACAAATCT
GGCGACAGGCGGGAATTAAACACTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15205.20 Da
• Isoelectric Point: 4.5036

>AT70604 WP_023315016.1 NZ_CP018814:3862747-3863154 [Enterobacter cloacae complex sp. AR_0002]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDEMLPLPGMVEAHLASHPEDFIGGQWL
LVDINMKQFEGRVERINITIPRRLLVKIDSFVSEHPQFTNRSAFLAEAARRVLPR

Nucleotide

• Length: 408 bp

>AT70604 NZ_CP018814:3862747-3863154 [Enterobacter cloacae complex sp. AR_0002]
ATGTTTTATCCGGCTTACATTCATTCTGATTCCGACGGTAGCGCCAGTGGTTTCTTTCCTGACGTTCCGGGGTGTTTTTT
TGCGGGGGATTCACTGGATGATGCTTTTCAGGATGCACGTGATGCATTAACTGCCCATTTTGAAGCGTTGTTTGAAATGG
ATGAAATGTTGCCACTTCCGGGTATGGTTGAAGCGCATCTGGCAAGTCATCCGGAGGATTTCATTGGCGGACAATGGTTG
CTGGTGGATATCAATATGAAACAGTTTGAAGGCAGAGTAGAGCGCATCAATATTACGATACCTCGCCGTTTGCTGGTGAA
GATTGATTCCTTTGTCAGCGAGCATCCACAATTTACTAACCGAAGTGCTTTTCTTGCAGAGGCAGCACGGCGTGTACTGC
CCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A4Q4AAS0