Detailed information of TA system TA070603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3352265..3352861
• Replicon: chromosome
• Accession: NZ_CP018814
• Organism: Enterobacter cloacae complex sp. AR_0002

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: AM329_RS16635
• Protein ID: WP_023315213.1
• Coordinates: 3352265..3352567 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: AM329_RS16640
• Protein ID: WP_032610152.1
• Coordinates: 3352574..3352861 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM329_RS16615 (AM329_16100)	3348484..3349833	+	1350	WP_032608241.1	lysine-sensitive aspartokinase 3	-
AM329_RS16620 (AM329_16105)	3349927..3350871	+	945	WP_032608239.1	ketopantoate/pantoate/pantothenate transporter PanS	-
AM329_RS16625 (AM329_16110)	3350922..3351194	+	273	WP_003860346.1	DUF3811 domain-containing protein	-
AM329_RS16630 (AM329_16115)	3351195..3352067	-	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
AM329_RS16635 (AM329_16120)	3352265..3352567	+	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM329_RS16640 (AM329_16125)	3352574..3352861	+	288	WP_032610152.1	putative addiction module antidote protein	Antitoxin
AM329_RS16645 (AM329_16130)	3352858..3354489	-	1632	WP_032610151.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T70603 WP_023315213.1 NZ_CP018814:3352265-3352567 [Enterobacter cloacae complex sp. AR_0002]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T70603 NZ_CP018814:3352265-3352567 [Enterobacter cloacae complex sp. AR_0002]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10072.60 Da
• Isoelectric Point: 5.7807

>AT70603 WP_032610152.1 NZ_CP018814:3352574-3352861 [Enterobacter cloacae complex sp. AR_0002]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT70603 NZ_CP018814:3352574-3352861 [Enterobacter cloacae complex sp. AR_0002]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8