Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2424584..2425204 Replicon chromosome
Accession NZ_CP018814
Organism Enterobacter cloacae complex sp. AR_0002

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag AM329_RS12010 Protein ID WP_015571250.1
Coordinates 2424986..2425204 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag AM329_RS12005 Protein ID WP_006809850.1
Coordinates 2424584..2424958 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM329_RS11995 (AM329_11645) 2419711..2420904 + 1194 WP_022650498.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM329_RS12000 (AM329_11650) 2420927..2424073 + 3147 WP_022650497.1 multidrug efflux RND transporter permease subunit AcrB -
AM329_RS12005 (AM329_11655) 2424584..2424958 + 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
AM329_RS12010 (AM329_11660) 2424986..2425204 + 219 WP_015571250.1 HHA domain-containing protein Toxin
AM329_RS12015 (AM329_11665) 2425413..2425964 + 552 WP_022650496.1 maltose O-acetyltransferase -
AM329_RS12020 (AM329_11670) 2426081..2426548 + 468 WP_022650495.1 YlaC family protein -
AM329_RS12025 (AM329_11675) 2426520..2427981 - 1462 Protein_2323 PLP-dependent aminotransferase family protein -
AM329_RS12030 (AM329_11680) 2428083..2428793 + 711 WP_022650493.1 GNAT family protein -
AM329_RS12035 (AM329_11685) 2428790..2428930 - 141 WP_032608666.1 type B 50S ribosomal protein L36 -
AM329_RS12040 (AM329_11690) 2428933..2429193 - 261 WP_015571255.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T70601 WP_015571250.1 NZ_CP018814:2424986-2425204 [Enterobacter cloacae complex sp. AR_0002]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T70601 NZ_CP018814:2424986-2425204 [Enterobacter cloacae complex sp. AR_0002]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT70601 WP_006809850.1 NZ_CP018814:2424584-2424958 [Enterobacter cloacae complex sp. AR_0002]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT70601 NZ_CP018814:2424584-2424958 [Enterobacter cloacae complex sp. AR_0002]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References