Detailed information of TA system TA070570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 461648..462380
• Replicon: chromosome
• Accession: NZ_CP018802
• Organism: Histophilus somni strain USDA-ARS-USMARC-63250

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: BTV18_RS02365
• Protein ID: WP_075294106.1
• Coordinates: 461913..462380 (+)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: BTV18_RS02360
• Protein ID: WP_011609927.1
• Coordinates: 461648..461911 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTV18_RS02330	456756..457118	+	363	WP_135026582.1	hypothetical protein	-
BTV18_RS02335	457181..459469	+	2289	WP_075321885.1	hypothetical protein	-
BTV18_RS09630	460135..460791	+	657	WP_157665518.1	hypothetical protein	-
BTV18_RS02350	460945..461199	+	255	WP_075294108.1	DUF1778 domain-containing protein	-
BTV18_RS02355	461199..461510	+	312	WP_075294107.1	hypothetical protein	-
BTV18_RS02360	461648..461911	+	264	WP_011609927.1	DUF1778 domain-containing protein	Antitoxin
BTV18_RS02365	461913..462380	+	468	WP_075294106.1	GNAT family N-acetyltransferase	Toxin
BTV18_RS02370	462730..462963	-	234	WP_011609925.1	hypothetical protein	-
BTV18_RS02375	463059..463361	-	303	WP_075294105.1	DUF2442 domain-containing protein	-
BTV18_RS02380	463330..463584	-	255	WP_075294104.1	DUF4160 domain-containing protein	-
BTV18_RS02385	463716..463976	-	261	Protein_456	hemophilus-specific protein	-
BTV18_RS02390	464053..464469	-	417	WP_075319814.1	IS200/IS605 family transposase	-
BTV18_RS02395	464516..465652	+	1137	WP_075319813.1	transposase	-
BTV18_RS02400	465908..466837	+	930	WP_075293400.1	hypothetical protein	-
BTV18_RS02405	466856..467302	-	447	WP_075293401.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	464053..464469	416

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-86)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17344.33 Da
• Isoelectric Point: 10.5585

>T70570 WP_075294106.1 NZ_CP018802:461913-462380 [Histophilus somni]
MYSAPELLTHHHHLGAFNCGEESLNIWLKRHALRNRQNRASRTFVICNENVVVGFYALAAGSVTHRFVSGALRRNMPEPI
PVVVLGRLAIDIKHQGKKLGAALLKDVVLRARAVSEQVGVRALLVHALNDKAKQFYLNYGFQPSPIDDMLLMLKL

Nucleotide

• Length: 468 bp

>T70570 NZ_CP018802:461913-462380 [Histophilus somni]
ATGTATTCAGCACCTGAGCTATTAACACATCATCACCATTTAGGTGCATTTAATTGTGGTGAAGAGTCATTAAATATTTG
GCTTAAACGTCATGCACTTAGAAACCGGCAAAACCGAGCAAGTAGAACATTTGTGATCTGCAATGAAAATGTCGTTGTTG
GCTTCTATGCACTAGCGGCAGGTTCAGTGACGCATCGGTTTGTATCCGGAGCATTAAGACGTAATATGCCTGAGCCTATT
CCTGTTGTTGTTTTAGGAAGATTAGCTATAGATATAAAACATCAAGGAAAAAAATTAGGTGCTGCATTACTTAAAGATGT
TGTACTACGAGCAAGAGCTGTGTCAGAACAAGTTGGAGTAAGAGCTTTGCTCGTACACGCTTTAAATGATAAAGCAAAAC
AATTCTATCTCAATTATGGTTTTCAACCTTCACCAATTGATGATATGTTATTGATGTTGAAGTTATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9906.35 Da
• Isoelectric Point: 5.6388

>AT70570 WP_011609927.1 NZ_CP018802:461648-461911 [Histophilus somni]
MRTAAINLRVQPKQRDLIDYAASLLGKTRSDFMLDVACTYAQNVILDRTVFQLDEGKFNHFIEVLEQPNLPNAGLEKLMA
THSPWES

Nucleotide

• Length: 264 bp

>AT70570 NZ_CP018802:461648-461911 [Histophilus somni]
ATGCGTACCGCTGCAATTAACCTACGAGTCCAGCCAAAGCAACGTGACTTAATTGATTATGCCGCTTCATTACTCGGAAA
AACACGTTCTGATTTTATGCTTGATGTTGCTTGCACTTATGCACAAAACGTGATTTTAGACAGAACAGTTTTTCAGCTTG
ATGAAGGAAAATTCAATCATTTTATTGAAGTACTTGAACAGCCTAATTTACCTAATGCCGGGTTGGAAAAATTAATGGCA
ACTCATTCACCTTGGGAATCATAA