Detailed information of TA system TA070562

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3770578..3771257
• Replicon: chromosome
• Accession: NZ_CP018801
• Organism: Escherichia coli strain tolC-

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: BTD92_RS20315
• Protein ID: WP_000854672.1
• Coordinates: 3770916..3771257 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: BTD92_RS20310
• Protein ID: WP_000070395.1
• Coordinates: 3770578..3770895 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTD92_RS20265	3765625..3766488	+	864	WP_001065553.1	GTPase family protein	-
BTD92_RS20270	3766580..3767401	+	822	WP_000197389.1	DUF945 domain-containing protein	-
BTD92_RS26450	3767618..3767809	+	192	Protein_3612	DeoR family transcriptional regulator	-
BTD92_RS26455	3767805..3768032	+	228	WP_001548158.1	hypothetical protein	-
BTD92_RS26460	3768032..3768475	+	444	WP_000824223.1	hypothetical protein	-
BTD92_RS20285	3768498..3768965	+	468	WP_001547765.1	hypothetical protein	-
BTD92_RS20290	3769042..3769281	+	240	WP_000194654.1	DUF905 domain-containing protein	-
BTD92_RS20295	3769379..3769837	+	459	WP_000211838.1	antirestriction protein	-
BTD92_RS20300	3769853..3770329	+	477	WP_000811693.1	RadC family protein	-
BTD92_RS20305	3770338..3770559	+	222	WP_000691994.1	DUF987 domain-containing protein	-
BTD92_RS20310	3770578..3770895	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
BTD92_RS20315	3770916..3771257	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
BTD92_RS20325	3771829..3773082	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
BTD92_RS20330	3773094..3774197	-	1104	WP_001285288.1	glutamate 5-kinase	-
BTD92_RS20335	3774485..3775540	+	1056	WP_000749863.1	phosphoporin PhoE	-
BTD92_RS20340	3775579..3775980	-	402	WP_000174689.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T70562 WP_000854672.1 NZ_CP018801:3770916-3771257 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T70562 NZ_CP018801:3770916-3771257 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT70562 WP_000070395.1 NZ_CP018801:3770578-3770895 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT70562 NZ_CP018801:3770578-3770895 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684