Detailed information of TA system TA070561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3553877..3554495
• Replicon: chromosome
• Accession: NZ_CP018801
• Organism: Escherichia coli strain tolC-

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: BTD92_RS19055
• Protein ID: WP_001290581.1
• Coordinates: 3554277..3554495 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: BTD92_RS19050
• Protein ID: WP_000344800.1
• Coordinates: 3553877..3554251 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTD92_RS19040	3548966..3550159	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
BTD92_RS19045	3550182..3553331	+	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
BTD92_RS19050	3553877..3554251	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
BTD92_RS19055	3554277..3554495	+	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
BTD92_RS19060	3554667..3555218	+	552	WP_000102564.1	maltose O-acetyltransferase	-
BTD92_RS19065	3555334..3555804	+	471	WP_000136192.1	YlaC family protein	-
BTD92_RS19070	3555968..3557518	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
BTD92_RS19075	3557560..3557913	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
BTD92_RS19085	3558292..3558603	+	312	WP_000409911.1	MGMT family protein	-
BTD92_RS19090	3558634..3559206	-	573	WP_000779842.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T70561 WP_001290581.1 NZ_CP018801:3554277-3554495 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T70561 NZ_CP018801:3554277-3554495 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT70561 WP_000344800.1 NZ_CP018801:3553877-3554251 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT70561 NZ_CP018801:3553877-3554251 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5