Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3420944..3421469 Replicon chromosome
Accession NZ_CP018801
Organism Escherichia coli strain tolC-

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag BTD92_RS18360 Protein ID WP_001159868.1
Coordinates 3420944..3421249 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag BTD92_RS18365 Protein ID WP_000813634.1
Coordinates 3421251..3421469 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTD92_RS18345 3416908..3418074 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
BTD92_RS18350 3418662..3419417 - 756 WP_000852146.1 replication initiation protein RepE -
BTD92_RS18355 3420137..3420943 - 807 WP_000016982.1 site-specific integrase -
BTD92_RS18360 3420944..3421249 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
BTD92_RS18365 3421251..3421469 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BTD92_RS18370 3422049..3423137 + 1089 WP_000952217.1 transcriptional repressor PifC -
BTD92_RS18375 3423139..3425364 + 2226 WP_000698737.1 phage T7 exclusion protein -
BTD92_RS18380 3425414..3426313 - 900 WP_000963206.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - entA / entB / entE / entC / fepB / entS / fepD / fepG / fepC / entF / fes / fepA / entD 3254871..3444860 189989


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T70560 WP_001159868.1 NZ_CP018801:c3421249-3420944 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T70560 NZ_CP018801:c3421249-3420944 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT70560 WP_000813634.1 NZ_CP018801:c3421469-3421251 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT70560 NZ_CP018801:c3421469-3421251 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References