Detailed information of TA system TA070531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1502214..1502830
• Replicon: chromosome
• Accession: NZ_CP018796
• Organism: Lentilactobacillus parabuchneri strain FAM21731

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: FAM21731_RS06865
• Protein ID: WP_057910635.1
• Coordinates: 1502648..1502830 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FAM21731_RS06860
• Protein ID: WP_057910636.1
• Coordinates: 1502214..1502612 (-)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FAM21731_RS06840	1498038..1498952	+	915	WP_057910640.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FAM21731_RS06845	1499009..1499782	+	774	WP_057910639.1	4-hydroxy-tetrahydrodipicolinate reductase	-
FAM21731_RS06850	1499804..1500991	+	1188	WP_057910638.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
FAM21731_RS06855	1501012..1502091	+	1080	WP_057910637.1	aspartate-semialdehyde dehydrogenase	-
FAM21731_RS06860	1502214..1502612	-	399	WP_057910636.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FAM21731_RS06865	1502648..1502830	-	183	WP_057910635.1	type II toxin-antitoxin system HicA family toxin	Toxin
FAM21731_RS06870	1503003..1503857	+	855	WP_057910634.1	metallophosphoesterase	-
FAM21731_RS06875	1504287..1506686	+	2400	Protein_1311	phosphoketolase family protein	-
FAM21731_RS06880	1506808..1507566	+	759	WP_075141188.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-113)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7053.28 Da
• Isoelectric Point: 10.8513

>T70531 WP_057910635.1 NZ_CP018796:c1502830-1502648 [Lentilactobacillus parabuchneri]
MDSRTIIQMLKHDGWYKVRIHGDHWHFKHPQKLGIVTVPHPRKDIPKGTYNSILKQAGLK

Nucleotide

• Length: 183 bp

>T70531 NZ_CP018796:c1502830-1502648 [Lentilactobacillus parabuchneri]
ATGGACTCCAGAACCATCATCCAGATGTTAAAACATGATGGGTGGTACAAAGTTAGAATCCATGGGGACCATTGGCATTT
TAAACACCCTCAAAAGCTGGGGATTGTAACTGTGCCACATCCTCGTAAAGATATTCCAAAAGGAACGTACAATAGCATTC
TAAAACAAGCGGGGCTCAAATAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.02 Da
• Isoelectric Point: 6.2197

>AT70531 WP_057910636.1 NZ_CP018796:c1502612-1502214 [Lentilactobacillus parabuchneri]
MKYLYYALFKKQSDDKIEVTFPDLAPHAATYGTTMSDALHMAHDALEGYLLVVEDLHEKLPKASSPESLTHDTSQLIIPI
EVDTTLAREREDNVSIKKTLTIPKYLNDLGKQKSINFSATLTDALKHKLNIL

Nucleotide

• Length: 399 bp

>AT70531 NZ_CP018796:c1502612-1502214 [Lentilactobacillus parabuchneri]
ATGAAATATTTGTATTATGCTTTATTCAAAAAGCAATCAGATGACAAAATTGAAGTTACATTCCCTGACTTAGCCCCCCA
CGCAGCAACATATGGAACAACGATGTCAGATGCCCTTCATATGGCTCATGATGCCTTGGAAGGCTACTTATTAGTTGTTG
AGGATTTACACGAGAAGCTGCCTAAGGCTTCAAGTCCAGAATCATTAACACATGATACAAGCCAGCTGATCATTCCTATC
GAGGTCGACACAACGTTGGCACGGGAACGTGAAGACAATGTAAGTATCAAAAAGACGTTAACAATTCCAAAATATTTAAA
TGATTTAGGCAAGCAAAAAAGCATCAATTTCTCTGCCACACTCACGGATGCCTTAAAGCACAAGCTCAATATTTTATAA