Detailed information of TA system TA070530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 20241..21129
• Replicon: plasmid pVIC12175-1
• Accession: NZ_CP018794
• Organism: Campylobacter sp. RM12175

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: -
• Locus tag: CVIC12175_RS08390
• Protein ID: WP_086257348.1
• Coordinates: 20473..21129 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: -
• Locus tag: CVIC12175_RS08385
• Protein ID: WP_086257349.1
• Coordinates: 20241..20489 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVIC12175_RS08370	15380..17569	+	2190	WP_086257351.1	DUF1738 domain-containing protein	-
CVIC12175_RS08375	17579..19405	+	1827	WP_086257350.1	type IV secretory system conjugative DNA transfer family protein	-
CVIC12175_RS08380	19417..20124	+	708	WP_002850984.1	conjugal transfer protein TraL	-
CVIC12175_RS08385	20241..20489	+	249	WP_086257349.1	hypothetical protein	Antitoxin
CVIC12175_RS08390	20473..21129	+	657	WP_086257348.1	Fic family protein	Toxin
CVIC12175_RS08395	21190..21648	+	459	WP_086257347.1	S26 family signal peptidase	-
CVIC12175_RS08400	21645..22016	+	372	WP_086257346.1	conjugal transfer protein TraM	-
CVIC12175_RS08405	22127..22813	+	687	WP_086257345.1	AAA family ATPase	-
CVIC12175_RS08410	22817..23026	+	210	WP_040126369.1	hypothetical protein	-
CVIC12175_RS08415	23101..24663	-	1563	WP_086257344.1	relaxase/mobilization nuclease domain-containing protein	-
CVIC12175_RS08420	24638..25006	-	369	WP_086257343.1	DNA transfer protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..25220	25220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (50-159)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25217.90 Da
• Isoelectric Point: 9.9201

>T70530 WP_086257348.1 NZ_CP018794:20473-21129 [Campylobacter sp. RM12175]
MKENNEELFKRLITDNKLGIKDEKLFDKESRFYTGLRERELTLNPIKGNFDYQHLKNIHKHIFQDVFHWAGKDRMEVGLH
GNFGKYAPNGTITNFVPGKDLNATAQQIFTWLKEDNYLKNSKDLNDFAKNLAEFARNLNSLHPFREGNGRTQRIFLNELA
KNAGYKLDLNLIPKDKMIMASVEASQLKLGKLEALIKTNLKSFKQNLEKENTLGLSLC

Nucleotide

• Length: 657 bp

>T70530 NZ_CP018794:20473-21129 [Campylobacter sp. RM12175]
ATGAAAGAAAACAATGAAGAACTTTTTAAAAGATTAATTACAGATAATAAACTCGGTATAAAAGATGAAAAATTATTTGA
TAAAGAATCAAGATTTTATACTGGTTTAAGAGAAAGAGAGCTTACGCTCAATCCTATCAAAGGCAATTTCGATTATCAGC
ACTTAAAAAATATACATAAACATATTTTTCAAGATGTATTTCACTGGGCTGGAAAAGATAGAATGGAAGTAGGCTTGCAT
GGAAATTTTGGAAAATATGCACCAAATGGAACAATTACTAATTTTGTTCCTGGAAAAGATTTAAATGCAACCGCTCAACA
AATTTTTACTTGGCTTAAAGAAGACAATTACTTAAAAAATTCAAAAGATCTTAACGATTTTGCAAAGAACTTAGCCGAAT
TTGCTAGAAACCTAAATTCTTTGCACCCCTTTAGAGAAGGTAACGGCAGAACTCAAAGAATATTTTTAAATGAATTGGCT
AAAAATGCAGGTTATAAACTTGATTTAAATTTAATACCAAAAGATAAAATGATAATGGCATCTGTAGAAGCTTCACAATT
GAAATTAGGAAAACTAGAAGCCTTAATAAAAACAAATCTAAAAAGCTTTAAGCAAAATTTAGAAAAAGAAAATACATTAG
GCTTATCCCTATGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9528.86 Da
• Isoelectric Point: 4.5686

>AT70530 WP_086257349.1 NZ_CP018794:20241-20489 [Campylobacter sp. RM12175]
MTYPEVHSLEESLAILKKYKDDVNKEDYEGIKSVISNHAIESIYMNELDIIAMIKQSALKLSADEIIAEYKEKGFVEYER
KQ

Nucleotide

• Length: 249 bp

>AT70530 NZ_CP018794:20241-20489 [Campylobacter sp. RM12175]
ATGACATATCCAGAAGTGCATAGTTTAGAAGAAAGTTTAGCAATACTTAAAAAATATAAAGATGATGTAAATAAGGAAGA
TTATGAAGGTATAAAATCTGTTATAAGTAATCATGCGATAGAGAGTATTTACATGAATGAATTAGATATTATCGCTATGA
TAAAACAAAGTGCTTTAAAGTTAAGTGCTGATGAAATCATTGCTGAATATAAGGAAAAGGGATTTGTAGAATATGAAAGA
AAACAATGA