Detailed information of TA system TA070529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 19977..20865
• Replicon: plasmid pVIC8964
• Accession: NZ_CP018792
• Organism: Campylobacter sp. RM8964

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: -
• Locus tag: CVIC8964_RS09010
• Protein ID: WP_086334268.1
• Coordinates: 20209..20865 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: -
• Locus tag: CVIC8964_RS09005
• Protein ID: WP_086257349.1
• Coordinates: 19977..20225 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVIC8964_RS08990	15116..17305	+	2190	WP_086334265.1	DUF1738 domain-containing protein	-
CVIC8964_RS08995	17315..19141	+	1827	WP_086334266.1	type IV secretory system conjugative DNA transfer family protein	-
CVIC8964_RS09000	19153..19860	+	708	WP_086334267.1	conjugal transfer protein TraL	-
CVIC8964_RS09005	19977..20225	+	249	WP_086257349.1	hypothetical protein	Antitoxin
CVIC8964_RS09010	20209..20865	+	657	WP_086334268.1	Fic family protein	Toxin
CVIC8964_RS09015	20926..21381	+	456	WP_086334269.1	S26 family signal peptidase	-
CVIC8964_RS09020	21378..21749	+	372	WP_086334270.1	conjugal transfer protein TraM	-
CVIC8964_RS09025	21860..22546	+	687	WP_086334271.1	AAA family ATPase	-
CVIC8964_RS09030	22550..22759	+	210	WP_040126369.1	hypothetical protein	-
CVIC8964_RS09035	22839..24401	-	1563	WP_086334272.1	relaxase/mobilization nuclease domain-containing protein	-
CVIC8964_RS09040	24376..24744	-	369	WP_086334273.1	DNA transfer protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..24958	24958

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (50-159)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25208.85 Da
• Isoelectric Point: 9.8126

>T70529 WP_086334268.1 NZ_CP018792:20209-20865 [Campylobacter sp. RM8964]
MKENNEEFFKRLITDNKLGIKDEKLFDKESRFYTGLRERELTLNPIKGNFDYQHLKNIHKHIFQDVFHWAGKDRMEVGLH
GNFGKYAPNGTITNFVPGKDLNATAQQIFTWLKEDNYLTNSKDLNDFAKNLAEFARNLNALHPFREGNGRTQRIFLNELA
KNAGYKLDLNLIPKDKMIMASVEASQLKLGKLEAIIKTNLKSFKQNLEKENTLGLSLC

Nucleotide

• Length: 657 bp

>T70529 NZ_CP018792:20209-20865 [Campylobacter sp. RM8964]
ATGAAAGAAAACAATGAAGAATTTTTTAAAAGATTAATTACAGATAATAAACTCGGTATAAAAGATGAAAAATTATTTGA
TAAAGAATCAAGATTTTATACTGGTTTAAGAGAAAGAGAGCTTACGCTCAATCCTATCAAAGGCAATTTCGATTATCAGC
ACTTAAAAAATATACATAAACATATTTTTCAAGATGTATTTCACTGGGCTGGAAAAGATAGAATGGAAGTAGGCTTGCAT
GGAAATTTTGGAAAATATGCACCAAATGGAACAATTACTAATTTTGTTCCTGGAAAAGATTTAAATGCAACCGCTCAACA
AATTTTTACTTGGCTTAAAGAAGACAATTACTTAACAAATTCAAAAGATCTTAACGATTTTGCAAAGAACTTAGCCGAAT
TTGCTAGAAACCTAAATGCTTTGCACCCCTTTAGAGAAGGTAACGGCAGAACTCAAAGAATATTTTTAAATGAATTGGCT
AAAAATGCAGGTTATAAACTTGATTTAAATTTAATACCAAAAGATAAAATGATAATGGCATCTGTAGAAGCTTCACAATT
GAAATTAGGAAAACTTGAAGCCATAATAAAAACAAATCTAAAAAGCTTTAAGCAAAATTTAGAAAAAGAAAATACATTAG
GCTTATCCCTATGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9528.86 Da
• Isoelectric Point: 4.5686

>AT70529 WP_086257349.1 NZ_CP018792:19977-20225 [Campylobacter sp. RM8964]
MTYPEVHSLEESLAILKKYKDDVNKEDYEGIKSVISNHAIESIYMNELDIIAMIKQSALKLSADEIIAEYKEKGFVEYER
KQ

Nucleotide

• Length: 249 bp

>AT70529 NZ_CP018792:19977-20225 [Campylobacter sp. RM8964]
ATGACATATCCAGAAGTGCATAGTTTAGAAGAAAGTTTAGCAATACTTAAAAAATATAAAGATGATGTAAATAAGGAAGA
TTATGAAGGTATAAAATCTGTTATAAGTAATCATGCGATAGAGAGTATTTACATGAATGAATTAGATATTATCGCTATGA
TAAAACAAAGTGCTTTAAAGTTAAGTGCTGATGAAATCATTGCTGAATATAAGGAAAAGGGATTTGTAGAATATGAAAGA
AAACAATGA