Detailed information of TA system TA070528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1867983..1869460
• Replicon: chromosome
• Accession: NZ_CP018787
• Organism: Oxalobacter formigenes strain HC-1

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: C3X8C7
• Locus tag: BRW83_RS09005
• Protein ID: WP_005879940.1
• Coordinates: 1867983..1869239 (-)
• Length: 419 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: BRW83_RS09010
• Protein ID: WP_005879938.1
• Coordinates: 1869236..1869460 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRW83_RS08990	1864748..1865233	-	486	WP_036602666.1	hypothetical protein	-
BRW83_RS08995	1866176..1867042	+	867	WP_005879944.1	hypothetical protein	-
BRW83_RS09000	1867099..1867974	+	876	WP_005879942.1	hypothetical protein	-
BRW83_RS09005	1867983..1869239	-	1257	WP_005879940.1	type II toxin-antitoxin system HipA family toxin	Toxin
BRW83_RS09010	1869236..1869460	-	225	WP_005879938.1	helix-turn-helix transcriptional regulator	Antitoxin
BRW83_RS09015	1869649..1870026	-	378	WP_036602664.1	DUF1622 domain-containing protein	-
BRW83_RS09020	1870289..1870936	+	648	WP_036603576.1	NAD(P)H-binding protein	-
BRW83_RS09025	1870948..1871403	+	456	WP_036602661.1	pyridoxamine 5'-phosphate oxidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (146-351)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-66)

Sequences

Toxin

Protein

• Length: 419 a.a.
• Molecular weight: 46365.63 Da
• Isoelectric Point: 6.7525

>T70528 WP_005879940.1 NZ_CP018787:c1869239-1867983 [Oxalobacter formigenes]
MSRILDVYLHEKMAGTLAQNENGQLVFKYNSNYLDSNGMAISVSMPLRDEPYPDRIARPYFAGLLPDERARRRLAAVLGV
SDENVFGLLEIIGGECAGALSLLPQGQPLPDFSTGTVEVLDGKRLAELLDLLRQKPLLGGVEGIRLSLAGAQDKLAVCFI
DGQIFLPKNGLPTTHILKPFIEGLSGTVENEVFCMMLAGRVGLDAPVVSKGMAGNTGFILVERYDRVREENGRILRLHQE
DFCQALGVPPEIKYESEGGPGIASSQALIQNVTQRPAADRRAFQQRVIFHYLVGNADAHAKNYSLLYQGKTPVLAPVYDV
VCTDVYSLAKNMAMKIGERSLPDTIRLEDWQKMVPDTRVAKRILSDDLLRMANGIEIEADRLLEELNDQNLYHPVLKKIR
RTIGVRVRTIQKLMEKIA

Nucleotide

• Length: 1257 bp

>T70528 NZ_CP018787:c1869239-1867983 [Oxalobacter formigenes]
ATGAGTCGCATTCTTGATGTTTACCTTCATGAAAAGATGGCGGGTACGCTGGCGCAAAATGAGAATGGCCAACTTGTTTT
CAAATATAACAGTAATTATCTGGATTCAAACGGGATGGCAATTTCAGTATCGATGCCTTTACGTGATGAGCCTTACCCGG
ACAGGATCGCTCGTCCGTACTTTGCCGGTTTGTTGCCGGACGAACGTGCGCGACGTCGTCTTGCGGCTGTGTTGGGTGTT
TCTGATGAAAATGTTTTCGGTTTGCTGGAGATAATCGGTGGTGAATGTGCAGGTGCACTTTCCCTGTTACCGCAAGGACA
ACCGTTGCCCGATTTTTCTACCGGAACTGTCGAGGTACTCGACGGGAAACGTCTTGCTGAACTGCTGGACCTGTTACGGC
AAAAACCATTGCTTGGAGGTGTGGAAGGAATTCGCTTATCTCTTGCCGGGGCACAGGATAAACTGGCCGTTTGTTTCATT
GACGGGCAAATTTTTTTGCCCAAAAACGGACTTCCGACCACACATATTCTCAAACCTTTTATCGAAGGATTGAGCGGAAC
GGTTGAAAACGAGGTTTTCTGTATGATGCTGGCGGGGCGCGTGGGATTGGATGCGCCTGTCGTTTCCAAAGGCATGGCAG
GAAATACCGGATTCATTCTTGTTGAACGTTATGACCGTGTGCGCGAAGAGAATGGCAGGATTTTACGCTTGCATCAGGAA
GATTTCTGTCAGGCTCTGGGTGTGCCGCCGGAAATTAAATATGAATCGGAAGGTGGGCCGGGAATAGCCAGTTCACAGGC
GTTGATCCAGAATGTAACGCAGAGGCCGGCTGCCGATCGTCGTGCTTTTCAGCAAAGGGTGATTTTTCATTATCTGGTGG
GAAACGCGGATGCTCATGCCAAAAATTATTCCCTGCTATATCAGGGCAAGACGCCCGTTCTGGCTCCGGTTTACGATGTC
GTATGTACAGATGTATATTCTCTTGCAAAGAATATGGCCATGAAAATTGGTGAGCGCTCTCTGCCAGATACCATACGATT
GGAAGACTGGCAAAAAATGGTACCGGACACCCGGGTGGCAAAAAGGATATTGTCTGATGATTTATTACGAATGGCGAATG
GTATAGAAATCGAAGCGGACAGGCTTCTGGAAGAATTGAACGACCAAAATCTTTATCATCCGGTGTTGAAAAAAATCAGG
AGAACGATCGGTGTCCGGGTGCGAACGATTCAAAAGCTTATGGAAAAAATTGCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8124.44 Da
• Isoelectric Point: 10.5957

>AT70528 WP_005879938.1 NZ_CP018787:c1869460-1869236 [Oxalobacter formigenes]
MTSVQNHIALGNLVRIERKRQKLTQEQLAALAGVGVRFLRELEKGKDSCRIGLAFSVMQTLGLSVDVSGRGDEK

Nucleotide

• Length: 225 bp

>AT70528 NZ_CP018787:c1869460-1869236 [Oxalobacter formigenes]
ATGACTTCAGTTCAAAATCATATTGCACTTGGCAATCTTGTTCGTATCGAACGGAAGCGTCAGAAGTTGACACAGGAGCA
ACTGGCTGCGCTTGCCGGTGTGGGTGTCCGGTTTTTACGTGAGCTGGAAAAAGGAAAGGACAGTTGTCGAATCGGTCTTG
CGTTTTCTGTCATGCAAACCCTTGGGTTGTCTGTGGATGTTTCCGGAAGAGGAGACGAAAAATGA

Structures

Toxin

• AlphaFold DB: C3X8C7