Detailed information of TA system TA070526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1437905..1438486
• Replicon: chromosome
• Accession: NZ_CP018787
• Organism: Oxalobacter formigenes strain HC-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BRW83_RS06830
• Protein ID: WP_086131780.1
• Coordinates: 1437905..1438198 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BRW83_RS06835
• Protein ID: WP_086131781.1
• Coordinates: 1438202..1438486 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRW83_RS06805	1433065..1433424	-	360	WP_086131775.1	hypothetical protein	-
BRW83_RS06815	1433747..1436260	-	2514	WP_086131777.1	hypothetical protein	-
BRW83_RS06820	1436253..1437098	-	846	WP_086131778.1	KilA-N domain-containing protein	-
BRW83_RS06825	1437091..1437813	-	723	WP_086131779.1	hypothetical protein	-
BRW83_RS06830	1437905..1438198	+	294	WP_086131780.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BRW83_RS06835	1438202..1438486	+	285	WP_086131781.1	putative addiction module antidote protein	Antitoxin
BRW83_RS06840	1438513..1438908	-	396	WP_086131782.1	winged helix-turn-helix domain-containing protein	-
BRW83_RS11695	1438905..1439045	-	141	WP_157661014.1	hypothetical protein	-
BRW83_RS06850	1439212..1439670	-	459	WP_086131784.1	hypothetical protein	-
BRW83_RS11870	1439696..1439920	-	225	WP_186447524.1	hypothetical protein	-
BRW83_RS06860	1439975..1440364	+	390	WP_198341921.1	helix-turn-helix domain-containing protein	-
BRW83_RS06865	1440573..1441103	+	531	WP_145814966.1	hypothetical protein	-
BRW83_RS11700	1441112..1441252	+	141	WP_157661015.1	hypothetical protein	-
BRW83_RS06870	1441256..1441435	+	180	WP_086131788.1	hypothetical protein	-
BRW83_RS11875	1441445..1442221	+	777	WP_198341922.1	DUF3310 domain-containing protein	-
BRW83_RS06880	1442211..1442570	+	360	WP_086131789.1	DUF4406 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1409703..1447782	38079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (2-90)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10925.66 Da
• Isoelectric Point: 10.8032

>T70526 WP_086131780.1 NZ_CP018787:1437905-1438198 [Oxalobacter formigenes]
MKYTIKQTETFKTWLKTVKDRTAFIAIIRRLERAGNGNFGDAKSINDGVSEMRVDVGAGYRLYYTIRGNEIVFFLNGGKK
STQKSDIKIAKSKAKGI

Nucleotide

• Length: 294 bp

>T70526 NZ_CP018787:1437905-1438198 [Oxalobacter formigenes]
ATGAAATACACAATCAAACAAACCGAAACATTTAAAACGTGGCTCAAAACAGTCAAGGATAGAACGGCATTTATTGCCAT
CATCAGAAGACTTGAAAGAGCCGGAAACGGCAATTTCGGGGATGCCAAGTCGATTAATGACGGTGTTTCTGAAATGCGCG
TCGATGTCGGTGCAGGTTATCGCTTGTATTACACCATTCGCGGAAATGAGATCGTGTTTTTCCTGAACGGTGGAAAAAAG
TCCACACAGAAATCGGATATTAAAATCGCAAAAAGCAAGGCGAAAGGAATTTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10617.06 Da
• Isoelectric Point: 5.1514

>AT70526 WP_086131781.1 NZ_CP018787:1438202-1438486 [Oxalobacter formigenes]
MTEKTHTFDIVEYLDSEENVQEYFRQVMEDGDSDEIIRALGHIAKARGMMKLANDTGLARESLYRALSEGAKPRFDTIMR
VTQALGLKLTIAHG

Nucleotide

• Length: 285 bp

>AT70526 NZ_CP018787:1438202-1438486 [Oxalobacter formigenes]
ATGACTGAAAAAACACACACTTTTGACATAGTTGAATATCTGGACAGTGAAGAAAACGTCCAGGAATATTTCAGACAAGT
CATGGAAGACGGCGACTCCGATGAAATTATCCGGGCGCTCGGCCACATCGCCAAAGCGCGGGGCATGATGAAACTTGCAA
ATGATACCGGACTCGCCAGAGAAAGCCTTTACAGGGCGCTTTCTGAAGGCGCAAAACCGCGTTTTGACACGATTATGCGC
GTCACTCAAGCCCTTGGCCTGAAACTGACTATTGCGCACGGCTGA