70514

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 294117..294710 Replicon plasmid pOAAA2
Accession NZ_CP018781
Organism Brucella pituitosa strain AA2

Toxin (Protein)


Gene name PumA Uniprot ID -
Locus tag BUE85_RS17630 Protein ID WP_079212851.1
Coordinates 294117..294380 (+) Length 88 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag BUE85_RS17635 Protein ID WP_079212852.1
Coordinates 294417..294710 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BUE85_RS25885 289458..289568 + 111 Protein_263 integrase core domain-containing protein -
BUE85_RS17590 289823..290857 - 1035 WP_152027379.1 IS110 family transposase -
BUE85_RS17595 290925..291101 + 177 Protein_265 transposase -
BUE85_RS17600 291241..292004 + 764 Protein_266 IS5 family transposase -
BUE85_RS25890 292274..292690 - 417 WP_152027380.1 hypothetical protein -
BUE85_RS17605 292746..293105 - 360 WP_079212847.1 metalloregulator ArsR/SmtB family transcription factor -
BUE85_RS17615 293370..293570 - 201 WP_079212849.1 hypothetical protein -
BUE85_RS25895 293602..293972 + 371 Protein_270 Tn3 family transposase -
BUE85_RS17630 294117..294380 + 264 WP_079212851.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BUE85_RS17635 294417..294710 + 294 WP_079212852.1 putative addiction module antidote protein Antitoxin
BUE85_RS17640 296068..296348 - 281 Protein_273 integrase core domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..332144 332144


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-86)

Antitoxin

Cro/C1-type helix-turn-helix domain

(45-86)


Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 10121.74 Da        Isoelectric Point: 9.5433

>T70514 WP_079212851.1 NZ_CP018781:294117-294380 [Brucella pituitosa]
MFSVRETIEFSNWLLRLRDDRAKTRIMQRIDRLARGNPGDMKLLGDGLAELRFDYGPGYRVYYIRAGEVLILLLCGGDKR
SQDKDIL

Download         Length: 264 bp

>T70514 NZ_CP018781:294117-294380 [Brucella pituitosa]
ATGTTTTCCGTACGCGAAACTATTGAATTTTCGAATTGGCTTTTAAGGCTACGAGACGACCGTGCTAAGACGCGTATCAT
GCAGCGAATTGACCGTCTAGCGCGTGGAAATCCGGGAGACATGAAGCTTCTTGGTGACGGACTTGCCGAGCTGCGCTTTG
ATTATGGACCTGGATATAGGGTTTACTACATTCGCGCTGGAGAAGTTCTGATCCTGCTCCTTTGCGGCGGAGATAAGCGC
TCACAGGACAAGGATATTTTGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10232.66 Da        Isoelectric Point: 5.0492

>AT70514 WP_079212852.1 NZ_CP018781:294417-294710 [Brucella pituitosa]
MPITTREYDPARYLNSEDAISEYIALASESGDPHELAMALGVVAKARGITEVARQTGLSRQAIYKALDVDGNPALGTISK
VADALGFKLSLVPKTAA

Download         Length: 294 bp

>AT70514 NZ_CP018781:294417-294710 [Brucella pituitosa]
ATGCCCATTACAACACGAGAATACGATCCAGCGCGCTATCTTAATAGTGAAGACGCTATTTCTGAATACATTGCGCTCGC
AAGCGAAAGTGGTGATCCACATGAACTTGCCATGGCTTTAGGTGTGGTAGCAAAGGCGCGAGGAATAACTGAAGTGGCGA
GACAGACGGGCCTTAGCCGTCAGGCAATTTATAAAGCGCTTGATGTCGACGGTAACCCAGCTTTAGGGACAATCTCGAAA
GTTGCTGATGCGCTCGGCTTCAAGCTTTCACTTGTACCTAAAACAGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References