Detailed information of TA system TA070512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 52162..52757
• Replicon: plasmid pOAAA2
• Accession: NZ_CP018781
• Organism: Brucella pituitosa strain AA2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: BUE85_RS16385
• Protein ID: WP_079212664.1
• Coordinates: 52162..52464 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BUE85_RS16390
• Protein ID: WP_079212871.1
• Coordinates: 52464..52757 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUE85_RS16365	48632..49834	-	1203	WP_079212662.1	transposase	-
BUE85_RS16370	49871..50252	+	382	Protein_43	IS200/IS605 family transposase	-
BUE85_RS16380	51329..52110	+	782	Protein_44	DUF945 domain-containing protein	-
BUE85_RS16385	52162..52464	+	303	WP_079212664.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BUE85_RS16390	52464..52757	+	294	WP_079212871.1	putative addiction module antidote protein	Antitoxin
BUE85_RS16395	52991..53754	-	764	Protein_47	IS5 family transposase	-
BUE85_RS16400	54090..54452	-	363	WP_079212665.1	copper resistance protein CopC	-
BUE85_RS16405	55140..56672	+	1533	WP_079212872.1	malonyl-CoA synthase	-
BUE85_RS16410	56800..57093	-	294	WP_079212666.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..332144	332144
	-	flank	IS/Tn	-	-	53326..53754	428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-95)

Antitoxin

• Uncharacterized protein HI1420 (7-93)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11546.35 Da
• Isoelectric Point: 10.5429

>T70512 WP_079212664.1 NZ_CP018781:52162-52464 [Brucella pituitosa]
MNVFQRSDLFDRWLKSLKDQRARLRILHRLTAAEKGNFGDVEPVGQGVSEMRLHYGSGYRIYYTRKEATIYLLLVGGDKS
SQKRDIKKAIEMARAVGKEL

Nucleotide

• Length: 303 bp

>T70512 NZ_CP018781:52162-52464 [Brucella pituitosa]
ATGAACGTATTCCAGAGATCAGATTTATTTGATCGGTGGCTTAAATCTCTAAAAGATCAAAGAGCTCGTCTCCGTATCTT
GCATCGATTAACTGCTGCTGAAAAGGGCAATTTTGGGGATGTTGAACCTGTTGGACAAGGTGTCTCGGAAATGCGCCTCC
ATTACGGGTCAGGGTATAGGATTTACTATACTCGAAAAGAGGCGACTATCTATCTGCTTTTGGTTGGCGGCGATAAGTCT
TCGCAGAAGCGAGATATTAAAAAGGCGATAGAAATGGCCAGAGCCGTCGGAAAGGAATTATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10548.06 Da
• Isoelectric Point: 4.4805

>AT70512 WP_079212871.1 NZ_CP018781:52464-52757 [Brucella pituitosa]
MTEFAPFDISEYLDNDEAMAEYLAIAMEDEDPDVFIAALGYVAKARGMASIAESAGLGRESLYKALKPGSKLRYETVQKV
LHALGFRLAVQSSLKAA

Nucleotide

• Length: 294 bp

>AT70512 NZ_CP018781:52464-52757 [Brucella pituitosa]
ATGACTGAATTTGCACCCTTTGACATTTCCGAATATCTCGACAACGACGAAGCCATGGCTGAGTATTTGGCTATTGCAAT
GGAAGATGAAGACCCCGATGTCTTTATTGCTGCTCTGGGATATGTCGCGAAAGCCCGAGGCATGGCGAGTATTGCGGAAT
CCGCAGGACTCGGTCGTGAGAGCCTGTATAAAGCTCTTAAACCCGGGTCCAAGCTACGCTATGAAACCGTTCAAAAGGTC
CTGCATGCACTTGGTTTCAGGCTCGCTGTGCAGTCGTCACTGAAAGCCGCTTAA