Detailed information of TA system TA070510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 425706..426253
• Replicon: chromosome
• Accession: NZ_CP018779
• Organism: Brucella pituitosa strain AA2

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BUE85_RS01870
• Protein ID: WP_079210780.1
• Coordinates: 426008..426253 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: BUE85_RS01865
• Protein ID: WP_079210779.1
• Coordinates: 425706..426008 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUE85_RS01845	420979..422478	+	1500	WP_079210777.1	IS21 family transposase	-
BUE85_RS01850	422480..423205	+	726	Protein_352	IS21-like element helper ATPase IstB	-
BUE85_RS01855	423520..424686	-	1167	WP_079210778.1	IS110 family transposase	-
BUE85_RS01860	425119..425709	+	591	WP_167377071.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BUE85_RS01865	425706..426008	-	303	WP_079210779.1	BrnA antitoxin family protein	Antitoxin
BUE85_RS01870	426008..426253	-	246	WP_079210780.1	BrnT family toxin	Toxin
BUE85_RS01875	426343..426723	+	381	Protein_357	hypothetical protein	-
BUE85_RS01880	426611..427030	+	420	WP_167377068.1	hypothetical protein	-
BUE85_RS01885	427270..427617	-	348	WP_079210782.1	hypothetical protein	-
BUE85_RS01890	427722..428810	+	1089	WP_079210783.1	hypothetical protein	-
BUE85_RS01895	428807..430027	+	1221	WP_161492393.1	MFS transporter	-
BUE85_RS01900	430024..431001	+	978	WP_079210785.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (25-98)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8986.51 Da
• Isoelectric Point: 8.8093

>T70510 WP_079210780.1 NZ_CP018779:c426253-426008 [Brucella pituitosa]
MKIVWDEPKREANLVKHGMDFASLTIDFFETSTVIPAKNGRLQAIGRLADGTIVVIFVTLGTEALSVISMRPARKDERSL
I

Nucleotide

• Length: 246 bp

>T70510 NZ_CP018779:c426253-426008 [Brucella pituitosa]
ATGAAGATCGTATGGGACGAACCAAAACGCGAGGCCAATCTTGTCAAGCACGGCATGGATTTTGCTTCGCTAACTATCGA
TTTTTTCGAAACCTCGACTGTAATTCCCGCCAAGAATGGCAGGCTTCAAGCTATTGGTCGCCTCGCAGACGGAACAATCG
TTGTTATCTTCGTGACCCTCGGCACAGAAGCGCTCTCCGTTATCTCAATGCGCCCTGCCCGAAAAGATGAAAGGAGCCTA
ATCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11358.12 Da
• Isoelectric Point: 10.4789

>AT70510 WP_079210779.1 NZ_CP018779:c426008-425706 [Brucella pituitosa]
MAIKFNPKHAAEHGYIKEDWDTVSDNPPITKEEMKKAKPFKEALPALYESIQRSRGRPKVANPKEAVTLRLDPDVVEKFK
ATGKNWRGRMNEALRKAAGL

Nucleotide

• Length: 303 bp

>AT70510 NZ_CP018779:c426008-425706 [Brucella pituitosa]
ATGGCTATCAAATTTAATCCGAAACATGCTGCAGAGCATGGCTACATCAAAGAAGATTGGGACACGGTTTCAGACAATCC
TCCTATCACCAAAGAGGAAATGAAAAAGGCCAAACCATTCAAGGAAGCGCTACCAGCGCTTTATGAAAGCATACAGCGCT
CACGCGGTCGGCCTAAGGTTGCCAACCCAAAAGAGGCGGTTACGCTGCGTCTTGATCCCGATGTTGTCGAAAAATTCAAG
GCAACGGGAAAAAACTGGCGCGGGAGGATGAACGAAGCACTACGCAAAGCCGCAGGCCTTTAA