Detailed information of TA system TA070509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 86135..86766
• Replicon: chromosome
• Accession: NZ_CP018779
• Organism: Brucella pituitosa strain AA2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BUE85_RS00405
• Protein ID: WP_079210536.1
• Coordinates: 86365..86766 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BUE85_RS00400
• Protein ID: WP_079210535.1
• Coordinates: 86135..86365 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUE85_RS00370	81736..82956	+	1221	WP_079210531.1	MFS transporter	-
BUE85_RS00375	82956..83342	+	387	Protein_75	VOC family protein	-
BUE85_RS00380	83463..83936	+	474	WP_079211254.1	carboxymuconolactone decarboxylase family protein	-
BUE85_RS00385	83983..84369	+	387	WP_079210532.1	hypothetical protein	-
BUE85_RS25980	84642..84782	-	141	WP_161492378.1	hypothetical protein	-
BUE85_RS00390	84902..85447	+	546	WP_079210533.1	hypothetical protein	-
BUE85_RS00395	85497..85859	+	363	WP_079210534.1	helix-turn-helix domain-containing protein	-
BUE85_RS00400	86135..86365	+	231	WP_079210535.1	antitoxin	Antitoxin
BUE85_RS00405	86365..86766	+	402	WP_079210536.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BUE85_RS00410	87239..88741	+	1503	WP_152027220.1	C69 family dipeptidase	-
BUE85_RS00420	89745..90725	+	981	WP_079210538.1	sugar ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	77528..86766	9238

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15085.30 Da
• Isoelectric Point: 6.7133

>T70509 WP_079210536.1 NZ_CP018779:86365-86766 [Brucella pituitosa]
MLKYMLDTNICIFTIKNRPQQVREAFNRFHDQLCISSISLMELIYGAEKSATPEKNLAVVEGFAARLEVLLYDEPAANHT
GQLRAELARDGTPIGPYDQLIAGHARSRGLIIVTNNRREFDRVPGLRVEDWTL

Nucleotide

• Length: 402 bp

>T70509 NZ_CP018779:86365-86766 [Brucella pituitosa]
ATGCTGAAGTATATGCTGGACACTAATATATGCATTTTTACGATCAAGAATAGACCACAGCAAGTGCGGGAAGCATTTAA
CCGCTTCCACGATCAATTATGCATTAGCTCGATTAGCCTGATGGAATTGATTTACGGGGCGGAAAAGTCCGCCACTCCCG
AAAAAAACCTTGCAGTAGTGGAGGGGTTCGCAGCTCGCCTCGAAGTGTTATTGTACGATGAACCGGCTGCAAATCATACG
GGTCAATTACGTGCCGAGTTGGCCCGTGATGGAACTCCTATCGGCCCATATGATCAATTGATAGCCGGCCACGCCCGCTC
ACGTGGTTTGATCATAGTTACGAACAATCGTAGAGAGTTTGATCGTGTTCCTGGCTTACGGGTAGAAGACTGGACCCTTT
AG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8510.65 Da
• Isoelectric Point: 4.5203

>AT70509 WP_079210535.1 NZ_CP018779:86135-86365 [Brucella pituitosa]
MEQGAVFQSNRSQAIRLPKAVALPADVKRVDIVAVGRTRIIAPVGEVWDSWFEGIEPTSDFMIERDQPTSQEREAL

Nucleotide

• Length: 231 bp

>AT70509 NZ_CP018779:86135-86365 [Brucella pituitosa]
ATGGAGCAGGGAGCGGTATTCCAGAGCAACAGAAGTCAAGCAATCCGCTTGCCAAAGGCAGTAGCTTTGCCTGCCGACGT
AAAGCGTGTTGATATCGTAGCTGTGGGGCGCACTCGAATTATTGCACCTGTTGGTGAGGTGTGGGATAGCTGGTTCGAAG
GAATTGAACCAACATCTGACTTCATGATTGAGCGTGATCAGCCTACGTCGCAGGAACGCGAGGCGTTGTAA