Detailed information of TA system TA070508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 77528..78075
• Replicon: chromosome
• Accession: NZ_CP018779
• Organism: Brucella pituitosa strain AA2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BUE85_RS00335
• Protein ID: WP_079210527.1
• Coordinates: 77782..78075 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: BUE85_RS00330
• Protein ID: WP_079210526.1
• Coordinates: 77528..77782 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUE85_RS00310	72907..74028	-	1122	WP_079211252.1	Fic family protein	-
BUE85_RS00315	74376..74753	-	378	WP_079210523.1	type II toxin-antitoxin system VapC family toxin	-
BUE85_RS00320	74750..74950	-	201	WP_079210524.1	type II toxin-antitoxin system VapB family antitoxin	-
BUE85_RS00325	75190..76731	-	1542	WP_079210525.1	Fic family protein	-
BUE85_RS25550	77004..77102	+	99	Protein_65	IS6 family transposase	-
BUE85_RS00330	77528..77782	+	255	WP_079210526.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BUE85_RS00335	77782..78075	+	294	WP_079210527.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BUE85_RS00340	78662..78922	+	261	WP_079210528.1	type II toxin-antitoxin system ParD family antitoxin	-
BUE85_RS00345	78925..79214	+	290	Protein_69	type II toxin-antitoxin system RelE/ParE family toxin	-
BUE85_RS00350	79351..79620	+	270	WP_152027218.1	DDE-type integrase/transposase/recombinase	-
BUE85_RS25975	79892..80059	+	168	Protein_71	transposase	-
BUE85_RS00360	80090..81007	-	918	WP_079210529.1	LysR family transcriptional regulator	-
BUE85_RS00365	81110..81736	+	627	WP_079210530.1	NAD(P)H:quinone oxidoreductase	-
BUE85_RS00370	81736..82956	+	1221	WP_079210531.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	77528..86766	9238
	-	inside	Prophage	-	-	60307..76731	16424
	-	flank	IS/Tn	-	-	77004..77126	122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Antitoxin ParD (8-70)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11166.87 Da
• Isoelectric Point: 5.1100

>T70508 WP_079210527.1 NZ_CP018779:77782-78075 [Brucella pituitosa]
MVLNLSVKAEEDIIAIAEYGIAMFGPLQAKRYHSDLFNTLDLIAKNPEMAREREEISPPVRIHPFKAHLIIYQSEQNGAI
YVIRIRNAFEDWISDPL

Nucleotide

• Length: 294 bp

>T70508 NZ_CP018779:77782-78075 [Brucella pituitosa]
ATGGTGCTTAATCTCTCTGTTAAGGCGGAAGAAGATATCATCGCTATTGCAGAGTACGGCATAGCAATGTTCGGACCTTT
ACAGGCCAAACGATATCATTCTGACCTGTTTAACACGTTGGATCTGATCGCCAAAAATCCAGAAATGGCACGTGAACGAG
AAGAAATCTCTCCGCCTGTCCGTATTCATCCGTTTAAAGCTCATCTGATTATTTATCAAAGTGAACAAAACGGAGCGATA
TATGTAATTAGGATACGGAATGCTTTTGAAGACTGGATTAGCGATCCTTTATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9518.85 Da
• Isoelectric Point: 10.4652

>AT70508 WP_079210526.1 NZ_CP018779:77528-77782 [Brucella pituitosa]
MATMNVSLPHPMKEWVEAQAKTGRYSNVSDYVRDLIRKDQMRGDKIAAMQRFVDEGLKSGKGTRSRDDLFATAISNAEKS
LKRN

Nucleotide

• Length: 255 bp

>AT70508 NZ_CP018779:77528-77782 [Brucella pituitosa]
ATGGCTACGATGAATGTATCGCTCCCTCATCCTATGAAAGAATGGGTTGAGGCCCAGGCCAAAACAGGACGCTATTCGAA
CGTAAGTGACTATGTGCGCGACTTGATCCGTAAGGATCAGATGCGTGGCGATAAGATCGCAGCAATGCAGCGATTTGTTG
ATGAAGGCCTAAAAAGTGGCAAGGGAACTCGTTCGCGGGATGATCTATTTGCTACGGCAATTTCTAACGCCGAAAAATCT
CTGAAACGCAACTAA