Detailed information of TA system TA070507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 74376..74950
• Replicon: chromosome
• Accession: NZ_CP018779
• Organism: Brucella pituitosa strain AA2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BUE85_RS00315
• Protein ID: WP_079210523.1
• Coordinates: 74376..74753 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BUE85_RS00320
• Protein ID: WP_079210524.1
• Coordinates: 74750..74950 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUE85_RS00300	70230..71135	+	906	WP_079210521.1	3-keto-5-aminohexanoate cleavage protein	-
BUE85_RS00305	71141..72619	+	1479	WP_079210522.1	carnitine 3-dehydrogenase	-
BUE85_RS00310	72907..74028	-	1122	WP_079211252.1	Fic family protein	-
BUE85_RS00315	74376..74753	-	378	WP_079210523.1	type II toxin-antitoxin system VapC family toxin	Toxin
BUE85_RS00320	74750..74950	-	201	WP_079210524.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BUE85_RS00325	75190..76731	-	1542	WP_079210525.1	Fic family protein	-
BUE85_RS25550	77004..77102	+	99	Protein_65	IS6 family transposase	-
BUE85_RS00330	77528..77782	+	255	WP_079210526.1	type II toxin-antitoxin system ParD family antitoxin	-
BUE85_RS00335	77782..78075	+	294	WP_079210527.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BUE85_RS00340	78662..78922	+	261	WP_079210528.1	type II toxin-antitoxin system ParD family antitoxin	-
BUE85_RS00345	78925..79214	+	290	Protein_69	type II toxin-antitoxin system RelE/ParE family toxin	-
BUE85_RS00350	79351..79620	+	270	WP_152027218.1	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	77528..86766	9238
	-	inside	Prophage	-	-	60307..76731	16424
	-	flank	IS/Tn	-	-	77004..77126	122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14045.29 Da
• Isoelectric Point: 5.9730

>T70507 WP_079210523.1 NZ_CP018779:c74753-74376 [Brucella pituitosa]
VILADTSIWIDHFRSGDNELIKIIADDLLLCHPAIIGELALGSLRDRTSVLAFLNVQREAQVATHDEVMTMIEMHCIFSM
GIGYTDAQLLASVLLDRRTSLWTRDKRLKITAEKAGARLYEPLHS

Nucleotide

• Length: 378 bp

>T70507 NZ_CP018779:c74753-74376 [Brucella pituitosa]
GTGATTTTGGCAGACACTTCAATTTGGATTGACCATTTCCGCAGCGGCGATAATGAGCTCATTAAAATTATCGCTGATGA
TCTTTTGCTTTGCCATCCAGCGATTATAGGCGAACTTGCGTTGGGGAGTCTCCGAGACAGGACGAGTGTATTGGCTTTTT
TGAATGTACAGCGTGAAGCTCAAGTCGCAACACATGACGAAGTCATGACCATGATTGAAATGCACTGCATTTTTAGCATG
GGCATTGGCTATACTGATGCCCAATTGCTGGCATCAGTTCTTCTCGACCGGCGAACTTCACTGTGGACCCGAGACAAGCG
CTTAAAGATTACGGCTGAAAAAGCCGGTGCACGGCTTTACGAGCCGCTGCACAGTTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7304.50 Da
• Isoelectric Point: 10.9054

>AT70507 WP_079210524.1 NZ_CP018779:c74950-74750 [Brucella pituitosa]
MRSTINLDDNLLEKARLLTGTKETAALVRQALETLVRLETGKRLIALGGTMPDVEAAPRRRSSEKK

Nucleotide

• Length: 201 bp

>AT70507 NZ_CP018779:c74950-74750 [Brucella pituitosa]
ATGCGCTCAACCATCAATCTTGATGACAATCTTTTAGAAAAAGCCAGATTGTTGACCGGAACGAAAGAGACAGCCGCTTT
AGTCCGACAAGCGCTAGAAACACTTGTGCGACTAGAGACTGGTAAACGCCTTATCGCTCTTGGAGGCACGATGCCGGACG
TTGAAGCAGCTCCTCGCCGTCGGAGCTCAGAGAAGAAGTGA