Detailed information of TA system TA070495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3070173..3070852
• Replicon: chromosome
• Accession: NZ_CP018778
• Organism: Mycobacterium tuberculosis strain DK9897

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: BTU11_RS15140
• Protein ID: WP_003414059.1
• Coordinates: 3070173..3070589 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: BTU11_RS15145
• Protein ID: WP_003414061.1
• Coordinates: 3070586..3070852 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTU11_RS15120	3066225..3067127	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BTU11_RS15125	3067196..3067948	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
BTU11_RS15130	3068192..3068467	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
BTU11_RS15135	3068464..3070086	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
BTU11_RS15140	3070173..3070589	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
BTU11_RS15145	3070586..3070852	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BTU11_RS15150	3070878..3071273	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
BTU11_RS15155	3071270..3071539	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
BTU11_RS15160	3071549..3072643	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
BTU11_RS15165	3072640..3073059	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
BTU11_RS15170	3073133..3073612	-	480	WP_003414073.1	dihydrofolate reductase	-
BTU11_RS15175	3073683..3074483	-	801	WP_009939104.1	thymidylate synthase	-
BTU11_RS15180	3074639..3075376	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T70495 WP_003414059.1 NZ_CP018778:c3070589-3070173 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T70495 NZ_CP018778:c3070589-3070173 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT70495 WP_003414061.1 NZ_CP018778:c3070852-3070586 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT70495 NZ_CP018778:c3070852-3070586 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64