Detailed information of TA system TA070465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1024218..1025191
• Replicon: chromosome
• Accession: NZ_CP018778
• Organism: Mycobacterium tuberculosis strain DK9897

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: O05911
• Locus tag: BTU11_RS04985
• Protein ID: WP_003404756.1
• Coordinates: 1024691..1025191 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: O05910
• Locus tag: BTU11_RS04980
• Protein ID: WP_003898647.1
• Coordinates: 1024218..1024694 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTU11_RS04960	1020065..1021336	-	1272	WP_003404747.1	PPE family protein	-
BTU11_RS04965	1021351..1021650	-	300	WP_003404748.1	PE family protein	-
BTU11_RS04970	1022094..1023875	+	1782	WP_003404749.1	BCCT family transporter	-
BTU11_RS04975	1023872..1024129	+	258	WP_003404750.1	hypothetical protein	-
BTU11_RS04980	1024218..1024694	+	477	WP_003898647.1	DUF1778 domain-containing protein	Antitoxin
BTU11_RS04985	1024691..1025191	+	501	WP_003404756.1	GNAT family N-acetyltransferase	Toxin
BTU11_RS04995	1025504..1026823	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
BTU11_RS05000	1027111..1027692	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
BTU11_RS05005	1027692..1029344	+	1653	WP_003404761.1	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T70465 WP_003404756.1 NZ_CP018778:1024691-1025191 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T70465 NZ_CP018778:1024691-1025191 [Mycobacterium tuberculosis]
GTGAGCGGCTACAGCGCGCCGCGACGTATCAGCGACGCCGATGACGTCACGAGCTTCAGCAGCGGCGAGCCCAGTCTGGA
CGATTACTTGCGCAAGCGGGCGTTGGCCAACCATGTGCAGGGAGGGTCGCGCTGTTTCGTGACGTGCCGTGACGGTCGGG
TAGTCGGCTTCTATGCGCTAGCGTCAGGGTCGGTCGCACACGCTGATGCTCCGGGACGGGTGCGCCGCAATATGCCTGAC
CCCGTGCCGGTGATCCTGCTGTCGCGGTTGGCGGTTGATCGCAAAGAACAGGGCAGGGGCCTGGGCAGTCATCTGCTGCG
TGATGCGATCGGTCGCTGTGTCCAGGCTGCGGACTCGATCGGGCTGCGGGCGATTCTTGTTCATGCGTTGCACGATGAGG
CCCGCGCGTTCTACGTCCACTTCGACTTCGAGATCTCGCCGACCGATCCGCTGCACCTAATGCTGTTGATGAAAGACGCT
CGCGCGCTAATTGGCGACTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17387.98 Da
• Isoelectric Point: 11.2467

>AT70465 WP_003898647.1 NZ_CP018778:1024218-1024694 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT70465 NZ_CP018778:1024218-1024694 [Mycobacterium tuberculosis]
TTGCACCGAGCGGGAGCGGCGGTGACGGCCAACGTGTGGTGTCGTGCGGGCGGCATTCGGATGGCGCCACGGCCGGTCAT
CCCGGTGGCTACGCAGCAGCGCCTGCGGCGGCAGGCGGATCGCCAGAGCCTGGGTAGTAGCGGCTTGCCAGCGTTGAATT
GTACGCCTATCAGGCACACAATTGATGTCATGGCTACCAAGCCTGAGCGGAAGACCGAGCGTCTTGCAGCGCGCCTGACC
CCTGAGCAGGACGCGCTGATTCGTCGTGCTGCCGAGGCCGAGGGGACTGACCTCACCAATTTCACGGTTACAGCGGCGTT
GGCGCACGCGCGCGACGTGCTGGCCGACCGCCGGCTCTTCGTACTCACCGATGCCGCGTGGACTGAGTTCCTCGCCGCGC
TGGACCGGCCCGTCTCACACAAGCCTCGGTTGGAGAAGCTGTTCGCCGCGCGGTCCATTTTCGACACCGAGGGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSZ2

Antitoxin

• AlphaFold DB: A5U0V8