Detailed information of TA system TA070454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 697152..697798
• Replicon: chromosome
• Accession: NZ_CP018778
• Organism: Mycobacterium tuberculosis strain DK9897

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BTU11_RS03185
• Protein ID: WP_031705732.1
• Coordinates: 697152..697565 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: BTU11_RS03190
• Protein ID: WP_003403139.1
• Coordinates: 697562..697798 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTU11_RS03165	693235..694785	+	1551	WP_003403119.1	MCE family protein	-
BTU11_RS03170	694837..695229	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
BTU11_RS03175	695226..695483	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
BTU11_RS03180	695666..696901	-	1236	WP_003403128.1	ATP-binding protein	-
BTU11_RS03185	697152..697565	-	414	WP_031705732.1	PIN domain-containing protein	Toxin
BTU11_RS03190	697562..697798	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BTU11_RS03195	697902..698408	-	507	WP_003900973.1	two component system sensor kinase HK1	-
BTU11_RS03200	698522..699052	-	531	WP_003917320.1	two component system sensor kinase HK2	-
BTU11_RS03205	699036..699797	-	762	WP_003403148.1	two-component system response regulator TcrA	-
BTU11_RS03210	699854..700165	+	312	WP_003403164.1	hypothetical protein	-
BTU11_RS03215	700237..701187	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
BTU11_RS03220	701428..702012	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
BTU11_RS03225	702014..702724	+	711	Protein_627	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14756.12 Da
• Isoelectric Point: 8.1405

>T70454 WP_031705732.1 NZ_CP018778:c697565-697152 [Mycobacterium tuberculosis]
VKPPLAVDASVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T70454 NZ_CP018778:c697565-697152 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACGCCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT70454 WP_003403139.1 NZ_CP018778:c697798-697562 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT70454 NZ_CP018778:c697798-697562 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Antitoxin

• AlphaFold DB: G0TQ89