Detailed information of TA system TA070402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 524194..524788
• Replicon: chromosome
• Accession: NZ_CP018762
• Organism: Microbacterium aurum strain KACC 15219

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: BOH66_RS02595
• Protein ID: WP_076689019.1
• Coordinates: 524477..524788 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BOH66_RS02590
• Protein ID: WP_076689017.1
• Coordinates: 524194..524493 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOH66_RS02575	519715..520920	+	1206	WP_076689011.1	AAA family ATPase	-
BOH66_RS02580	521017..522969	+	1953	WP_076689013.1	type I restriction-modification system subunit M	-
BOH66_RS02585	522966..524144	+	1179	WP_076689015.1	restriction endonuclease subunit S	-
BOH66_RS02590	524194..524493	-	300	WP_076689017.1	putative addiction module antidote protein	Antitoxin
BOH66_RS02595	524477..524788	-	312	WP_076689019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BOH66_RS02600	524870..527968	+	3099	WP_076689021.1	type I restriction endonuclease subunit R	-
BOH66_RS02605	528114..528608	-	495	WP_157127840.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	486637..545613	58976

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Uncharacterized protein HI1420 (8-95)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11730.36 Da
• Isoelectric Point: 8.9159

>T70402 WP_076689019.1 NZ_CP018762:c524788-524477 [Microbacterium aurum]
MVEVVKSAVFDQWIRKLKDRHARARVLVRIDRLAAGNPGDVKPVGDGISELRIDYGPGYRVYYLQEGSRLILLLCGGDKS
TQQSDITQAHRIAEEWKNHDRDV

Nucleotide

• Length: 312 bp

>T70402 NZ_CP018762:c524788-524477 [Microbacterium aurum]
ATGGTCGAGGTCGTCAAGAGCGCGGTCTTCGACCAGTGGATACGAAAACTCAAAGACCGGCACGCGCGAGCCCGCGTGCT
CGTCAGGATCGACCGGCTCGCCGCAGGCAACCCCGGCGACGTGAAACCGGTCGGCGACGGCATCTCCGAACTGAGAATCG
ACTACGGCCCCGGCTACCGGGTCTACTACCTCCAGGAAGGCTCGCGGCTCATCCTCCTGCTCTGCGGAGGCGACAAGTCC
ACCCAGCAGTCCGACATCACCCAGGCACACCGAATCGCAGAGGAGTGGAAGAACCATGACCGAGACGTCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10532.62 Da
• Isoelectric Point: 4.3402

>AT70402 WP_076689017.1 NZ_CP018762:c524493-524194 [Microbacterium aurum]
MTETSDRFSRYDTADYLTDPADAAAYLEAALEDSSDPTDVARALGTIARSGNLSELARRTGMSREGLYKALSADGNPSFA
TIVKVADALGLKLHFESVA

Nucleotide

• Length: 300 bp

>AT70402 NZ_CP018762:c524493-524194 [Microbacterium aurum]
ATGACCGAGACGTCTGACCGCTTCAGCCGTTACGACACGGCCGACTACCTCACCGACCCTGCCGACGCCGCCGCGTACCT
CGAGGCCGCGCTCGAAGACAGCAGCGACCCGACCGACGTCGCGCGTGCACTAGGCACCATCGCCCGATCCGGCAACCTCA
GCGAGCTCGCCCGCCGCACCGGCATGAGCCGCGAAGGCCTCTACAAAGCGCTCTCCGCCGACGGCAACCCCAGCTTCGCC
ACCATCGTGAAGGTCGCCGACGCCCTCGGCCTCAAGCTTCACTTCGAATCCGTCGCCTGA