Detailed information of TA system TA070400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PHD(antitoxin)
• Location: 255370..256012
• Replicon: chromosome
• Accession: NZ_CP018762
• Organism: Microbacterium aurum strain KACC 15219

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BOH66_RS01305
• Protein ID: WP_076688580.1
• Coordinates: 255629..256012 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BOH66_RS01300
• Protein ID: WP_076688578.1
• Coordinates: 255370..255627 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOH66_RS01285	251261..252775	-	1515	WP_084201871.1	MFS transporter	-
BOH66_RS01290	252853..254664	+	1812	WP_076688574.1	DEAD/DEAH box helicase	-
BOH66_RS01295	255014..255289	+	276	WP_076688576.1	helix-turn-helix domain-containing protein	-
BOH66_RS01300	255370..255627	+	258	WP_076688578.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
BOH66_RS01305	255629..256012	+	384	WP_076688580.1	type II toxin-antitoxin system VapC family toxin	Toxin
BOH66_RS01310	256506..258374	+	1869	WP_076688582.1	molecular chaperone DnaK	-
BOH66_RS01315	258387..258956	+	570	WP_076688584.1	nucleotide exchange factor GrpE	-
BOH66_RS01320	258965..259957	+	993	WP_076688586.1	DnaJ domain-containing protein	-
BOH66_RS01325	260078..260509	+	432	WP_076688588.1	MerR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-97)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13363.27 Da
• Isoelectric Point: 4.3905

>T70400 WP_076688580.1 NZ_CP018762:255629-256012 [Microbacterium aurum]
VIYLDTSAAVKALVDEPGSDLVRAVFAAGDPLVSSRLLRVELHAAVSRRWLEASGVDELLARVSLVGIDDDLLDRAVELG
SGLRALDSLHLATAVDLAADIEGMLGCDRELITAARREGIPPHPACG

Nucleotide

• Length: 384 bp

>T70400 NZ_CP018762:255629-256012 [Microbacterium aurum]
GTGATCTACCTCGACACGTCAGCGGCCGTGAAGGCGCTGGTCGACGAGCCGGGCTCCGACCTCGTCCGTGCCGTCTTCGC
GGCGGGAGATCCCCTCGTCTCGTCCCGCCTCCTCCGTGTCGAGCTGCACGCGGCCGTGAGTCGCCGATGGCTGGAGGCGT
CGGGTGTCGACGAGCTGCTGGCGCGTGTGTCGCTGGTCGGCATCGATGACGATCTCCTCGACCGCGCTGTGGAGCTCGGC
TCCGGACTGCGCGCGCTCGACTCCCTTCACCTCGCGACCGCGGTGGACCTGGCCGCGGACATCGAGGGCATGCTCGGCTG
CGATCGCGAGCTGATCACCGCCGCGCGCCGTGAAGGGATCCCTCCGCACCCCGCCTGCGGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9174.42 Da
• Isoelectric Point: 9.4590

>AT70400 WP_076688578.1 NZ_CP018762:255370-255627 [Microbacterium aurum]
MERVTRTELNQQTARVLARVAAGERIVITDRGRAIAQIAPVRESRWEQLVDAGRVQPATAAGALTVPPVRSASTAAEILD
DIRGE

Nucleotide

• Length: 258 bp

>AT70400 NZ_CP018762:255370-255627 [Microbacterium aurum]
ATGGAGCGCGTGACCAGGACCGAGTTGAACCAGCAGACCGCTCGCGTGCTCGCGCGCGTTGCGGCCGGTGAGCGCATCGT
CATCACCGACCGCGGCAGGGCAATCGCGCAGATCGCGCCCGTGCGCGAGTCGCGGTGGGAGCAGCTCGTGGATGCCGGCC
GGGTCCAGCCCGCCACAGCCGCCGGCGCTCTGACAGTCCCACCGGTTCGCAGCGCCTCCACCGCGGCGGAGATCCTCGAC
GACATCCGAGGCGAGTGA