Detailed information of TA system TA070392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 3024453..3025123
• Replicon: chromosome
• Accession: NZ_CP018758
• Organism: Pseudomonas psychrotolerans strain PRS08-11306

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BJP27_RS13630
• Protein ID: WP_058788273.1
• Coordinates: 3024453..3024872 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BJP27_RS13635
• Protein ID: WP_074528954.1
• Coordinates: 3024869..3025123 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJP27_RS13610	3019666..3019998	+	333	WP_058761747.1	hypothetical protein	-
BJP27_RS13615	3020039..3020992	-	954	WP_074528951.1	homoserine kinase	-
BJP27_RS13620	3021053..3021346	-	294	WP_058761749.1	DUF2782 domain-containing protein	-
BJP27_RS13625	3021457..3024204	+	2748	WP_074528952.1	DNA polymerase I	-
BJP27_RS13630	3024453..3024872	-	420	WP_058788273.1	type II toxin-antitoxin system VapC family toxin	Toxin
BJP27_RS13635	3024869..3025123	-	255	WP_074528954.1	Arc family DNA-binding protein	Antitoxin
BJP27_RS13640	3025407..3026180	-	774	WP_167371483.1	electron transfer flavoprotein subunit beta	-
BJP27_RS13645	3026197..3027432	-	1236	WP_074528958.1	electron transfer flavoprotein subunit alpha/FixB family protein	-
BJP27_RS13650	3027602..3029593	-	1992	WP_074528959.1	dimethylglycine demethylation protein DgcB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-41)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15188.61 Da
• Isoelectric Point: 7.7754

>T70392 WP_058788273.1 NZ_CP018758:c3024872-3024453 [Pseudomonas psychrotolerans]
MILLDTNVVSETMKPIPSPQVIAWLDRQAPATLYLSTITVAELQYGIACLPNSRRRDLLQQGVDALIRLYEGRILSFDLA
AACRYAELAAQARQRGLGFPLPDSYIGAIAAVSKFKIATRDEGPYLAAGLSIVNPWNTP

Nucleotide

• Length: 420 bp

>T70392 NZ_CP018758:c3024872-3024453 [Pseudomonas psychrotolerans]
ATGATTCTGCTCGATACCAACGTCGTCTCCGAGACGATGAAGCCAATCCCCTCGCCCCAAGTCATCGCTTGGTTGGATCG
CCAAGCGCCAGCAACGCTGTACCTGTCGACGATCACCGTTGCCGAATTGCAATATGGTATTGCCTGCCTGCCCAATAGCC
GTCGTCGCGACCTATTGCAGCAAGGGGTAGACGCCTTGATCCGGCTCTATGAGGGCCGGATTCTGAGCTTCGATCTGGCT
GCTGCCTGTCGTTATGCCGAATTGGCCGCACAGGCTCGCCAGCGTGGTCTTGGCTTTCCCCTGCCTGATTCCTACATAGG
CGCGATTGCAGCGGTCAGCAAATTCAAGATCGCCACTCGAGACGAGGGGCCTTACCTCGCCGCGGGGCTCAGCATCGTCA
ATCCCTGGAACACACCCTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9455.84 Da
• Isoelectric Point: 5.7152

>AT70392 WP_074528954.1 NZ_CP018758:c3025123-3024869 [Pseudomonas psychrotolerans]
MAQLTIRGLPEEVDRALRAQAARNGRSMEAEVRLILSQALILPTETPMGEAMAAIWRKPGITHEEQAFLEGTRDRRAHEP
MSFE

Nucleotide

• Length: 255 bp

>AT70392 NZ_CP018758:c3025123-3024869 [Pseudomonas psychrotolerans]
ATGGCTCAATTGACCATCCGTGGACTACCGGAAGAAGTCGATCGCGCGCTTCGCGCCCAGGCAGCTCGCAACGGGCGGAG
CATGGAGGCTGAGGTCAGACTCATCCTTAGCCAAGCCTTGATCTTGCCGACCGAGACGCCGATGGGAGAGGCCATGGCCG
CTATCTGGCGCAAACCCGGGATCACTCACGAAGAGCAGGCTTTCCTCGAGGGGACGCGTGACCGGCGCGCCCATGAGCCG
ATGTCATTCGAATGA