Detailed information of TA system TA070387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 32160..32803
• Replicon: plasmid pKp_Goe_641-1
• Accession: NZ_CP018737
• Organism: Klebsiella pneumoniae strain Kp_Goe_121641

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: BB745_RS29130
• Protein ID: WP_001044770.1
• Coordinates: 32160..32576 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: BB745_RS29135
• Protein ID: WP_001261282.1
• Coordinates: 32573..32803 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB745_RS29095	27514..28218	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BB745_RS29100	28295..29083	-	789	Protein_37	Tn3 family transposase	-
BB745_RS29105	29193..29897	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BB745_RS30555	29949..30038	-	90	Protein_39	recombinase family protein	-
BB745_RS29110	30202..30396	+	195	Protein_40	DUF4158 domain-containing protein	-
BB745_RS29115	30645..31342	-	698	Protein_41	IS1 family transposase	-
BB745_RS29125	31376..32086	-	711	WP_004152334.1	ATP-binding protein	-
BB745_RS29130	32160..32576	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
BB745_RS29135	32573..32803	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BB745_RS29140	32760..33221	+	462	WP_072202616.1	hypothetical protein	-
BB745_RS29145	33365..33715	+	351	WP_000493378.1	hypothetical protein	-
BB745_RS29150	33766..34509	+	744	WP_000129823.1	hypothetical protein	-
BB745_RS29155	34506..35282	+	777	WP_000015958.1	site-specific integrase	-
BB745_RS29160	35340..35597	-	258	WP_000764642.1	hypothetical protein	-
BB745_RS31205	35726..35830	-	105	WP_032409716.1	hypothetical protein	-
BB745_RS29175	36365..37231	+	867	WP_004118283.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / blaCTX-M-15 / dfrA14	-	1..72952	72952

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T70387 WP_001044770.1 NZ_CP018737:c32576-32160 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T70387 NZ_CP018737:c32576-32160 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT70387 WP_001261282.1 NZ_CP018737:c32803-32573 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT70387 NZ_CP018737:c32803-32573 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3