Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5400139..5400764 Replicon chromosome
Accession NZ_CP018735
Organism Klebsiella pneumoniae strain Kp_Goe_121641

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag BB745_RS28025 Protein ID WP_002882817.1
Coordinates 5400139..5400522 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag BB745_RS28030 Protein ID WP_004150355.1
Coordinates 5400522..5400764 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB745_RS28010 5397505..5398407 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
BB745_RS28015 5398404..5399039 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
BB745_RS28020 5399036..5399965 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
BB745_RS28025 5400139..5400522 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
BB745_RS28030 5400522..5400764 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
BB745_RS28035 5400969..5401886 + 918 WP_032418237.1 alpha/beta hydrolase -
BB745_RS28040 5401900..5402841 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
BB745_RS28045 5402886..5403323 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
BB745_RS28050 5403320..5404180 - 861 WP_004146232.1 virulence factor BrkB family protein -
BB745_RS28055 5404174..5404773 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T70386 WP_002882817.1 NZ_CP018735:c5400522-5400139 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T70386 NZ_CP018735:c5400522-5400139 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT70386 WP_004150355.1 NZ_CP018735:c5400764-5400522 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT70386 NZ_CP018735:c5400764-5400522 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References