Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 53542..54185 Replicon plasmid pKp_Goe_304-4
Accession NZ_CP018724
Organism Klebsiella pneumoniae strain KP_Goe_828304

Toxin (Protein)


Gene name vagD Uniprot ID A0A7Z7T700
Locus tag BB787_RS30900 Protein ID WP_040155119.1
Coordinates 53542..53958 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BB787_RS30905 Protein ID WP_001261276.1
Coordinates 53955..54185 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB787_RS30875 48987..49684 - 698 Protein_55 IS1 family transposase -
BB787_RS30885 49741..50694 - 954 Protein_56 colicin V synthesis protein -
BB787_RS30890 50684..51961 - 1278 Protein_57 HlyD family secretion protein -
BB787_RS30895 52623..53501 + 879 WP_009309916.1 restriction endonuclease -
BB787_RS30900 53542..53958 - 417 WP_040155119.1 type II toxin-antitoxin system VapC family toxin Toxin
BB787_RS30905 53955..54185 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BB787_RS30915 54759..55109 + 351 WP_000493378.1 hypothetical protein -
BB787_RS30920 55160..55903 + 744 WP_000129823.1 hypothetical protein -
BB787_RS30925 55900..56676 + 777 WP_000015958.1 site-specific integrase -
BB787_RS30930 56734..56991 - 258 WP_000764642.1 hypothetical protein -
BB787_RS33105 57120..57224 - 105 WP_032409716.1 hypothetical protein -
BB787_RS30945 57759..58625 + 867 WP_004118283.1 replication initiation protein -
BB787_RS30950 58802..59071 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(3'')-Ib / aph(6)-Id / blaCTX-M-15 / blaTEM-1B / qnrS1 / sul1 / qacE / dfrA1 / tet(A) - 1..61012 61012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15147.60 Da        Isoelectric Point: 7.8915

>T70361 WP_040155119.1 NZ_CP018724:c53958-53542 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIHLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T70361 NZ_CP018724:c53958-53542 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCATCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT70361 WP_001261276.1 NZ_CP018724:c54185-53955 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT70361 NZ_CP018724:c54185-53955 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7T700

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References