Detailed information of TA system TA070358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 28796..29706
• Replicon: plasmid pKp_Goe_304-1
• Accession: NZ_CP018720
• Organism: Klebsiella pneumoniae strain KP_Goe_828304

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: BB787_RS27890
• Protein ID: WP_004181896.1
• Coordinates: 29236..29706 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: BB787_RS27885
• Protein ID: WP_004181895.1
• Coordinates: 28796..29239 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB787_RS27865	25861..26076	-	216	Protein_38	IS200/IS605 family transposase	-
BB787_RS27870	26078..26647	+	570	Protein_39	IS200/IS605 family element transposase accessory protein TnpB	-
BB787_RS27875	27002..27424	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
BB787_RS27880	27424..28695	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
BB787_RS27885	28796..29239	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
BB787_RS27890	29236..29706	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
BB787_RS27895	29816..30076	-	261	WP_004026352.1	hypothetical protein	-
BB787_RS27900	30799..30957	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
BB787_RS33210	31560..32534	+	975	WP_064794367.1	hypothetical protein	-
BB787_RS27910	32732..33109	-	378	WP_004181901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..246757	246757
	-	flank	IS/Tn	-	-	26138..26647	509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T70358 WP_004181896.1 NZ_CP018720:29236-29706 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T70358 NZ_CP018720:29236-29706 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT70358 WP_004181895.1 NZ_CP018720:28796-29239 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT70358 NZ_CP018720:28796-29239 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9