Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 40501..41144 Replicon plasmid pKp_Goe_021-4
Accession NZ_CP018718
Organism Klebsiella pneumoniae strain Kp_Goe_152021

Toxin (Protein)


Gene name vagD Uniprot ID A0A7Z7T700
Locus tag BB786_RS30800 Protein ID WP_040155119.1
Coordinates 40728..41144 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BB786_RS30795 Protein ID WP_001261276.1
Coordinates 40501..40731 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB786_RS30750 35615..35884 + 270 WP_000339857.1 hypothetical protein -
BB786_RS30755 36061..36927 - 867 WP_004118283.1 replication initiation protein -
BB786_RS33030 37462..37566 + 105 WP_032409716.1 hypothetical protein -
BB786_RS30770 37695..37952 + 258 WP_000764642.1 hypothetical protein -
BB786_RS30775 38010..38786 - 777 WP_000015958.1 site-specific integrase -
BB786_RS30780 38783..39526 - 744 WP_000129823.1 hypothetical protein -
BB786_RS30785 39577..39927 - 351 WP_000493378.1 hypothetical protein -
BB786_RS30795 40501..40731 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BB786_RS30800 40728..41144 + 417 WP_040155119.1 type II toxin-antitoxin system VapC family toxin Toxin
BB786_RS30805 41185..42063 - 879 WP_009309916.1 restriction endonuclease -
BB786_RS30810 42725..44002 + 1278 Protein_46 HlyD family secretion protein -
BB786_RS30815 43992..44945 + 954 Protein_47 colicin V synthesis protein -
BB786_RS30825 45002..45699 + 698 Protein_48 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qnrS1 / blaTEM-1B / blaCTX-M-15 / aph(6)-Id / aph(3'')-Ib / tet(A) / dfrA1 / qacE / sul1 - 1..61010 61010


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15147.60 Da        Isoelectric Point: 7.8915

>T70338 WP_040155119.1 NZ_CP018718:40728-41144 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIHLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T70338 NZ_CP018718:40728-41144 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCATCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT70338 WP_001261276.1 NZ_CP018718:40501-40731 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT70338 NZ_CP018718:40501-40731 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7T700

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References