Detailed information of TA system TA070333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4959756..4961437
• Replicon: chromosome
• Accession: NZ_CP018713
• Organism: Klebsiella pneumoniae strain Kp_Goe_152021

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A6TH06
• Locus tag: BB786_RS25555
• Protein ID: WP_015959269.1
• Coordinates: 4959756..4960631 (-)
• Length: 292 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: BB786_RS25560
• Protein ID: WP_004177809.1
• Coordinates: 4960679..4961437 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB786_RS25535	4955062..4956582	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
BB786_RS25540	4956579..4957577	+	999	WP_002885172.1	ribose ABC transporter permease	-
BB786_RS25545	4957608..4958549	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
BB786_RS25550	4958624..4959631	+	1008	WP_015959270.1	carbohydrate kinase family protein	-
BB786_RS25555	4959756..4960631	-	876	WP_015959269.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
BB786_RS25560	4960679..4961437	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
BB786_RS25565	4961523..4962251	-	729	WP_032413197.1	response regulator transcription factor	-
BB786_RS25580	4962524..4964671	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
BB786_RS33135	4964668..4964811	-	144	WP_162897205.1	hypothetical protein	-
BB786_RS25585	4965249..4965935	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (7-264)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 292 a.a.
• Molecular weight: 32990.17 Da
• Isoelectric Point: 5.4637

>T70333 WP_015959269.1 NZ_CP018713:c4960631-4959756 [Klebsiella pneumoniae]
MQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQAGMSAVLQANRSDE
MRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQHPRDFYDVKLLLDAQ
ELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKSCITQQDVDFLLSFK
RGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 876 bp

>T70333 NZ_CP018713:c4960631-4959756 [Klebsiella pneumoniae]
ATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATTTGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGA
TTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGGTGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGG
ATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAGGCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAG
ATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAAGATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACC
ACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTTTTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCT
ACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCATCCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAG
GAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTATTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCC
TCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAATTCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGA
CAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCCTGTATCACACAACAGGATGTCGATTTTTTACTGTCATTTAAA
CGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCGTATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATAT
TCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAGATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT70333 WP_004177809.1 NZ_CP018713:c4961437-4960679 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT70333 NZ_CP018713:c4961437-4960679 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: A6TH06

Antitoxin

• AlphaFold DB: A6TH05