Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 26281..26924 Replicon plasmid pKp_Goe_026-4
Accession NZ_CP018711
Organism Klebsiella pneumoniae strain Kp_Goe_827026

Toxin (Protein)


Gene name vagD Uniprot ID A0A7Z7T700
Locus tag BB785_RS30270 Protein ID WP_040155119.1
Coordinates 26508..26924 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BB785_RS30265 Protein ID WP_001261276.1
Coordinates 26281..26511 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB785_RS30220 21395..21664 + 270 WP_000339857.1 hypothetical protein -
BB785_RS30225 21841..22707 - 867 WP_004118283.1 replication initiation protein -
BB785_RS33210 23242..23346 + 105 WP_032409716.1 hypothetical protein -
BB785_RS30240 23475..23732 + 258 WP_000764642.1 hypothetical protein -
BB785_RS30245 23790..24566 - 777 WP_000015958.1 site-specific integrase -
BB785_RS30250 24563..25306 - 744 WP_000129823.1 hypothetical protein -
BB785_RS30255 25357..25707 - 351 WP_000493378.1 hypothetical protein -
BB785_RS30265 26281..26511 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BB785_RS30270 26508..26924 + 417 WP_040155119.1 type II toxin-antitoxin system VapC family toxin Toxin
BB785_RS30275 26965..27843 - 879 WP_009309916.1 restriction endonuclease -
BB785_RS30280 28505..29782 + 1278 Protein_30 HlyD family secretion protein -
BB785_RS30285 29772..30725 + 954 Protein_31 colicin V synthesis protein -
BB785_RS30295 30782..31479 + 698 Protein_32 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / tet(A) / dfrA1 / qacE / sul1 / qnrS1 / blaTEM-1B / blaCTX-M-15 - 1..61009 61009


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15147.60 Da        Isoelectric Point: 7.8915

>T70315 WP_040155119.1 NZ_CP018711:26508-26924 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIHLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T70315 NZ_CP018711:26508-26924 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCATCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT70315 WP_001261276.1 NZ_CP018711:26281-26511 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT70315 NZ_CP018711:26281-26511 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7T700

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References