70290

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 134121..134643 Replicon plasmid pKp_Goe_024-1
Accession NZ_CP018702
Organism Klebsiella pneumoniae strain Kp_Goe_827024

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag BB784_RS28385 Protein ID WP_004181778.1
Coordinates 134359..134643 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag BB784_RS28380 Protein ID WP_004181777.1
Coordinates 134121..134369 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB784_RS28360 129330..130151 - 822 WP_004026535.1 hypothetical protein -
BB784_RS28365 130213..130566 - 354 WP_004181774.1 hypothetical protein -
BB784_RS28370 130711..131697 - 987 WP_040120328.1 hypothetical protein -
BB784_RS28375 132031..133830 - 1800 WP_038991636.1 ATP-dependent helicase -
BB784_RS28380 134121..134369 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BB784_RS28385 134359..134643 + 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BB784_RS31795 134660..134761 - 102 Protein_136 IS200/IS605 family transposase -
BB784_RS28390 134797..136020 + 1224 WP_004181779.1 transposase -
BB784_RS28395 136411..137598 - 1188 WP_004181780.1 transposase -
BB784_RS32940 138264..138731 + 468 WP_124737156.1 hypothetical protein -
BB784_RS32945 138776..138982 - 207 WP_063938351.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..246753 246753


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T70290 WP_004181778.1 NZ_CP018702:134359-134643 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T70290 NZ_CP018702:134359-134643 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT70290 WP_004181777.1 NZ_CP018702:134121-134369 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT70290 NZ_CP018702:134121-134369 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References