Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 231258..231780 Replicon plasmid pKp_Goe_832-1
Accession NZ_CP018696
Organism Klebsiella pneumoniae strain Kp_Goe_149832

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag BB751_RS28945 Protein ID WP_004181778.1
Coordinates 231496..231780 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag BB751_RS28940 Protein ID WP_004181777.1
Coordinates 231258..231506 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB751_RS28920 226467..227288 - 822 WP_004026535.1 hypothetical protein -
BB751_RS28925 227350..227703 - 354 WP_004181774.1 hypothetical protein -
BB751_RS28930 227848..228834 - 987 WP_040120328.1 hypothetical protein -
BB751_RS28935 229168..230967 - 1800 WP_038991636.1 ATP-dependent helicase -
BB751_RS28940 231258..231506 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BB751_RS28945 231496..231780 + 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BB751_RS31800 231797..231898 - 102 Protein_240 IS200/IS605 family transposase -
BB751_RS28950 231934..233157 + 1224 WP_004181779.1 transposase -
BB751_RS28955 233548..234735 - 1188 WP_004181780.1 transposase -
BB751_RS32960 235401..235868 + 468 WP_124737156.1 hypothetical protein -
BB751_RS32965 235913..236119 - 207 WP_063938351.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..246755 246755


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T70267 WP_004181778.1 NZ_CP018696:231496-231780 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T70267 NZ_CP018696:231496-231780 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT70267 WP_004181777.1 NZ_CP018696:231258-231506 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT70267 NZ_CP018696:231258-231506 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References