Detailed information of TA system TA070251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1474249..1474985
• Replicon: chromosome
• Accession: NZ_CP018695
• Organism: Klebsiella pneumoniae strain Kp_Goe_149832

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: BB751_RS07655
• Protein ID: WP_071836879.1
• Coordinates: 1474503..1474985 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: BB751_RS07650
• Protein ID: WP_003026799.1
• Coordinates: 1474249..1474515 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB751_RS07625	1469895..1471034	+	1140	WP_032433368.1	mannitol dehydrogenase	-
BB751_RS07630	1471063..1471725	+	663	WP_032433366.1	dihydroxyacetone kinase subunit L	-
BB751_RS07635	1471709..1472713	+	1005	WP_032433364.1	dihydroxyacetone kinase subunit DhaK	-
BB751_RS07640	1472731..1473363	+	633	WP_032433362.1	3-hexulose-6-phosphate synthase	-
BB751_RS07645	1473373..1473936	+	564	WP_032433361.1	6-phospho-3-hexuloisomerase	-
BB751_RS07650	1474249..1474515	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
BB751_RS07655	1474503..1474985	+	483	WP_071836879.1	GNAT family N-acetyltransferase	Toxin
BB751_RS07660	1475143..1476675	-	1533	WP_015065592.1	IS3-like element ISKpn38 family transposase	-
BB751_RS07665	1476787..1478190	+	1404	WP_039815096.1	ISNCY-like element ISKpn21 family transposase	-
BB751_RS07670	1478219..1478851	-	633	WP_001567369.1	hypothetical protein	-
BB751_RS07675	1479231..1479830	-	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1442194..1489813	47619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (80-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17481.06 Da
• Isoelectric Point: 9.8177

>T70251 WP_071836879.1 NZ_CP018695:1474503-1474985 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVYKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T70251 NZ_CP018695:1474503-1474985 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTACAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT70251 WP_003026799.1 NZ_CP018695:1474249-1474515 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT70251 NZ_CP018695:1474249-1474515 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66