Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 99053..99789 Replicon plasmid pKp_Goe_588-1
Accession NZ_CP018693
Organism Klebsiella pneumoniae strain Kp_Goe_821588

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag BB749_RS28355 Protein ID WP_003026803.1
Coordinates 99053..99535 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag BB749_RS28360 Protein ID WP_003026799.1
Coordinates 99523..99789 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB749_RS28335 94690..95652 + 963 WP_004152113.1 M48 family metalloprotease -
BB749_RS30120 95740..96994 + 1255 Protein_102 IS3 family transposase -
BB749_RS28350 97495..98841 - 1347 WP_020314316.1 ISNCY family transposase -
BB749_RS28355 99053..99535 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
BB749_RS28360 99523..99789 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
BB749_RS28365 99965..100219 - 255 WP_004152108.1 hypothetical protein -
BB749_RS28370 100295..100552 - 258 WP_004152107.1 hypothetical protein -
BB749_RS28375 100601..100804 - 204 WP_004152106.1 hemolysin expression modulator Hha -
BB749_RS28380 100838..101206 - 369 WP_004152105.1 hypothetical protein -
BB749_RS28385 101250..101744 - 495 WP_004152104.1 DNA-binding protein -
BB749_RS28390 101775..102350 - 576 WP_004152103.1 hypothetical protein -
BB749_RS28395 102338..102607 - 270 WP_004152102.1 hypothetical protein -
BB749_RS28400 102965..103315 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
BB749_RS28405 103365..103727 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / dfrA14 / aph(3')-Ia - 1..180027 180027


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T70246 WP_003026803.1 NZ_CP018693:c99535-99053 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T70246 NZ_CP018693:c99535-99053 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT70246 WP_003026799.1 NZ_CP018693:c99789-99523 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT70246 NZ_CP018693:c99789-99523 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References