Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 234842..235364 Replicon plasmid pKp_Goe_473-1
Accession NZ_CP018687
Organism Klebsiella pneumoniae strain Kp_Goe_149473

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag BB750_RS28935 Protein ID WP_004181778.1
Coordinates 234842..235126 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag BB750_RS28940 Protein ID WP_004181777.1
Coordinates 235116..235364 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB750_RS32940 230503..230709 + 207 WP_063938351.1 hypothetical protein -
BB750_RS32945 230754..231221 - 468 WP_124737156.1 hypothetical protein -
BB750_RS28925 231887..233074 + 1188 WP_004181780.1 transposase -
BB750_RS28930 233465..234688 - 1224 WP_004181779.1 transposase -
BB750_RS31780 234724..234825 + 102 Protein_235 IS200/IS605 family transposase -
BB750_RS28935 234842..235126 - 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BB750_RS28940 235116..235364 - 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BB750_RS28945 235655..237454 + 1800 WP_038991636.1 ATP-dependent helicase -
BB750_RS28950 237788..238774 + 987 WP_040120328.1 hypothetical protein -
BB750_RS28955 238919..239272 + 354 WP_004181774.1 hypothetical protein -
BB750_RS28960 239334..240155 + 822 WP_004026535.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..246757 246757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T70226 WP_004181778.1 NZ_CP018687:c235126-234842 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T70226 NZ_CP018687:c235126-234842 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT70226 WP_004181777.1 NZ_CP018687:c235364-235116 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT70226 NZ_CP018687:c235364-235116 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References