Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 9362..10128 Replicon plasmid p3
Accession NZ_CP018684
Organism Vibrio harveyi strain QT520

Toxin (Protein)


Gene name - Uniprot ID A0A8B3D766
Locus tag BG259_RS28400 Protein ID WP_017191060.1
Coordinates 9631..10128 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A347UQX1
Locus tag BG259_RS28395 Protein ID WP_017420534.1
Coordinates 9362..9634 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG259_RS28360 4656..4975 + 320 Protein_6 hypothetical protein -
BG259_RS28365 5163..5432 - 270 WP_017191054.1 Txe/YoeB family addiction module toxin -
BG259_RS28370 5425..5679 - 255 WP_017191055.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
BG259_RS28375 5831..6193 - 363 Protein_9 hypothetical protein -
BG259_RS28380 6197..7389 + 1193 Protein_10 IS91 family transposase -
BG259_RS28385 7588..8316 - 729 WP_074052119.1 hypothetical protein -
BG259_RS28390 8775..9134 - 360 WP_017191058.1 hypothetical protein -
BG259_RS28395 9362..9634 + 273 WP_017420534.1 DUF1778 domain-containing protein Antitoxin
BG259_RS28400 9631..10128 + 498 WP_017191060.1 GNAT family N-acetyltransferase Toxin
BG259_RS28405 10274..10702 - 429 WP_017819283.1 hypothetical protein -
BG259_RS28410 10737..10868 - 132 WP_074052120.1 DUF3265 domain-containing protein -
BG259_RS28415 10844..11131 - 288 WP_053050104.1 hypothetical protein -
BG259_RS28420 11269..11892 - 624 WP_172458673.1 DUF2971 domain-containing protein -
BG259_RS28425 12069..12254 - 186 WP_081384200.1 DUF3265 domain-containing protein -
BG259_RS28430 12217..12888 + 672 WP_186293500.1 hypothetical protein -
BG259_RS28435 12987..13823 - 837 WP_146219509.1 HNH endonuclease -
BG259_RS28440 14506..15072 - 567 WP_047517731.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..59857 59857


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18588.22 Da        Isoelectric Point: 7.3558

>T70205 WP_017191060.1 NZ_CP018684:9631-10128 [Vibrio harveyi]
MMNTVLLDKAKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDNDNSHVIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQEFEEAENKLFITIADV
RTSLG

Download         Length: 498 bp

>T70205 NZ_CP018684:9631-10128 [Vibrio harveyi]
ATGATGAATACGGTACTTCTAGATAAAGCTAAACACGATAGAAACCGATTCAACTGTGGTATCGAAGCTCTCAATAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCAAAAAAAGACAACACCAGAACGTTTGTTTTGGAAGATGATAACGACAACTCAC
ATGTCATCGGCTTTTACACGCTAACAATGACACCAATTGACTTAAAGGCATTACCCGATAAGTTACAAAAAAAACACCAA
TCATCCACCTCTGGTGGTCTGATTGCCCGCCTTGCTGTCGATGACCGATACAAAGGGAAAGGCTTTGGTGAGTGGCTGCT
TATTGACGCGCTCAGAAAGCTGTTAGCTGCAAGCGATAGCGTTGCATTTCCAGTTGTTATCGTTGATGCCAAAGACGGCG
CGAAACACTTCTATGAACGCTATGGTTTTCAAGAATTTGAAGAAGCTGAAAATAAACTCTTCATCACCATTGCCGATGTG
AGAACAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9735.22 Da        Isoelectric Point: 8.5960

>AT70205 WP_017420534.1 NZ_CP018684:9362-9634 [Vibrio harveyi]
MATTLPRITARVDVDTQDLLTKAAAIAGMSSINSFVLSAAIEKAKQVIEREQALKLSQADAMLLMEALDRPATQNSKLKA
AADRYESKTQ

Download         Length: 273 bp

>AT70205 NZ_CP018684:9362-9634 [Vibrio harveyi]
ATGGCTACTACTTTGCCTCGCATCACCGCTAGGGTTGATGTCGATACACAAGACTTACTTACTAAGGCTGCCGCGATCGC
CGGCATGTCTAGCATCAACTCGTTTGTTCTAAGCGCTGCAATCGAAAAAGCCAAACAAGTCATCGAACGCGAACAGGCTT
TAAAATTGAGCCAAGCTGATGCTATGTTGCTGATGGAAGCTTTAGACCGCCCTGCTACACAGAACTCAAAACTAAAAGCT
GCTGCTGATCGATACGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3D766

Antitoxin

Source ID Structure
AlphaFold DB A0A347UQX1

References