Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2746890..2747515 Replicon chromosome
Accession NZ_CP018676
Organism Klebsiella pneumoniae strain CAV1217

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag AGE86_RS15880 Protein ID WP_002882817.1
Coordinates 2747132..2747515 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AGE86_RS15875 Protein ID WP_004150355.1
Coordinates 2746890..2747132 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGE86_RS15850 2742881..2743480 + 600 WP_004151865.1 glucose-1-phosphatase -
AGE86_RS15855 2743474..2744334 + 861 WP_002882807.1 virulence factor BrkB family protein -
AGE86_RS15860 2744331..2744768 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AGE86_RS15865 2744813..2745754 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AGE86_RS15870 2745768..2746685 - 918 WP_002882812.1 alpha/beta hydrolase -
AGE86_RS15875 2746890..2747132 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AGE86_RS15880 2747132..2747515 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
AGE86_RS15885 2747689..2748618 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AGE86_RS15890 2748615..2749250 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
AGE86_RS15895 2749247..2750149 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T70192 WP_002882817.1 NZ_CP018676:2747132-2747515 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T70192 NZ_CP018676:2747132-2747515 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT70192 WP_004150355.1 NZ_CP018676:2746890-2747132 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT70192 NZ_CP018676:2746890-2747132 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References