Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 96675..97411 Replicon plasmid pKPC_CAV1217
Accession NZ_CP018675
Organism Klebsiella pneumoniae strain CAV1217

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AGE86_RS01465 Protein ID WP_003026803.1
Coordinates 96929..97411 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AGE86_RS01460 Protein ID WP_003026799.1
Coordinates 96675..96941 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGE86_RS01415 92737..93099 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
AGE86_RS01420 93149..93499 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AGE86_RS01425 93857..94126 + 270 WP_004152102.1 hypothetical protein -
AGE86_RS01430 94114..94689 + 576 WP_004152103.1 hypothetical protein -
AGE86_RS01435 94720..95214 + 495 WP_004152104.1 DNA-binding protein -
AGE86_RS01440 95258..95626 + 369 WP_004152105.1 hypothetical protein -
AGE86_RS01445 95660..95863 + 204 WP_004152106.1 hemolysin expression modulator Hha -
AGE86_RS01450 95912..96169 + 258 WP_004152107.1 hypothetical protein -
AGE86_RS01455 96245..96499 + 255 WP_004152108.1 hypothetical protein -
AGE86_RS01460 96675..96941 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AGE86_RS01465 96929..97411 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AGE86_RS01470 97623..98969 + 1347 WP_020314316.1 ISNCY family transposase -
AGE86_RS01475 99470..100724 - 1255 Protein_113 IS3 family transposase -
AGE86_RS01480 100812..101774 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-4 / aph(4)-Ia / aac(3)-IVa / sul1 / qacE - 1..181436 181436


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T70179 WP_003026803.1 NZ_CP018675:96929-97411 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T70179 NZ_CP018675:96929-97411 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT70179 WP_003026799.1 NZ_CP018675:96675-96941 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT70179 NZ_CP018675:96675-96941 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References