Detailed information of TA system TA070179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 96675..97411
• Replicon: plasmid pKPC_CAV1217
• Accession: NZ_CP018675
• Organism: Klebsiella pneumoniae strain CAV1217

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: AGE86_RS01465
• Protein ID: WP_003026803.1
• Coordinates: 96929..97411 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: AGE86_RS01460
• Protein ID: WP_003026799.1
• Coordinates: 96675..96941 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGE86_RS01415	92737..93099	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
AGE86_RS01420	93149..93499	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
AGE86_RS01425	93857..94126	+	270	WP_004152102.1	hypothetical protein	-
AGE86_RS01430	94114..94689	+	576	WP_004152103.1	hypothetical protein	-
AGE86_RS01435	94720..95214	+	495	WP_004152104.1	DNA-binding protein	-
AGE86_RS01440	95258..95626	+	369	WP_004152105.1	hypothetical protein	-
AGE86_RS01445	95660..95863	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
AGE86_RS01450	95912..96169	+	258	WP_004152107.1	hypothetical protein	-
AGE86_RS01455	96245..96499	+	255	WP_004152108.1	hypothetical protein	-
AGE86_RS01460	96675..96941	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
AGE86_RS01465	96929..97411	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
AGE86_RS01470	97623..98969	+	1347	WP_020314316.1	ISNCY family transposase	-
AGE86_RS01475	99470..100724	-	1255	Protein_113	IS3 family transposase	-
AGE86_RS01480	100812..101774	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-4 / aph(4)-Ia / aac(3)-IVa / sul1 / qacE	-	1..181436	181436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T70179 WP_003026803.1 NZ_CP018675:96929-97411 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T70179 NZ_CP018675:96929-97411 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT70179 WP_003026799.1 NZ_CP018675:96675-96941 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT70179 NZ_CP018675:96675-96941 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66