Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 35289..35812 Replicon plasmid pCAV1217-71
Accession NZ_CP018674
Organism Klebsiella pneumoniae strain CAV1217

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag AGE86_RS00590 Protein ID WP_015632464.1
Coordinates 35289..35594 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag AGE86_RS00595 Protein ID WP_015632465.1
Coordinates 35594..35812 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGE86_RS00545 30319..30747 - 429 WP_015632456.1 plasmid partitioning/stability family protein -
AGE86_RS00550 30740..31720 - 981 WP_015632457.1 partitioning protein -
AGE86_RS00555 32081..32263 + 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
AGE86_RS00560 32289..32732 + 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
AGE86_RS00565 32938..33060 - 123 Protein_38 transcriptional regulator -
AGE86_RS00570 33074..33301 - 228 WP_016162072.1 hypothetical protein -
AGE86_RS00575 33731..33988 + 258 WP_016162071.1 helix-turn-helix domain-containing protein -
AGE86_RS00580 34192..34758 - 567 WP_015632462.1 hypothetical protein -
AGE86_RS00585 34800..35144 - 345 WP_015632463.1 hypothetical protein -
AGE86_RS00590 35289..35594 - 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
AGE86_RS00595 35594..35812 - 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AGE86_RS00600 35944..36429 + 486 Protein_45 IS5 family transposase -
AGE86_RS00605 36593..37024 + 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
AGE86_RS00610 37024..38295 + 1272 WP_016162068.1 Y-family DNA polymerase -
AGE86_RS00615 38707..39582 + 876 WP_004098982.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..70606 70606


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T70178 WP_015632464.1 NZ_CP018674:c35594-35289 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T70178 NZ_CP018674:c35594-35289 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT70178 WP_015632465.1 NZ_CP018674:c35812-35594 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT70178 NZ_CP018674:c35812-35594 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References