Detailed information of TA system TA070174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3528373..3528889
• Replicon: chromosome
• Accession: NZ_CP018671
• Organism: Klebsiella pneumoniae strain CAV1042

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4YAY3
• Locus tag: AGE78_RS20455
• Protein ID: WP_004178374.1
• Coordinates: 3528605..3528889 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A919M0P8
• Locus tag: AGE78_RS20450
• Protein ID: WP_032434351.1
• Coordinates: 3528373..3528615 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGE78_RS20435	3524401..3525141	+	741	WP_004186692.1	KDGP aldolase family protein	-
AGE78_RS20440	3525208..3526362	+	1155	WP_004178372.1	lactonase family protein	-
AGE78_RS20445	3526385..3528295	+	1911	WP_031593171.1	BglG family transcription antiterminator	-
AGE78_RS20450	3528373..3528615	+	243	WP_032434351.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AGE78_RS20455	3528605..3528889	+	285	WP_004178374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AGE78_RS20460	3528893..3529357	-	465	WP_004178375.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
AGE78_RS20465	3529665..3531803	-	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
AGE78_RS20475	3532160..3532903	-	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
AGE78_RS31680	3532906..3533079	-	174	WP_019725541.1	hypothetical protein	-
AGE78_RS20485	3533164..3533472	+	309	WP_002886907.1	PTS sugar transporter subunit IIB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-68)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11155.97 Da
• Isoelectric Point: 10.3787

>T70174 WP_004178374.1 NZ_CP018671:3528605-3528889 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T70174 NZ_CP018671:3528605-3528889 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9003.47 Da
• Isoelectric Point: 6.3340

>AT70174 WP_032434351.1 NZ_CP018671:3528373-3528615 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGGKVSLDDL

Nucleotide

• Length: 243 bp

>AT70174 NZ_CP018671:3528373-3528615 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A6THG1