Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1026911..1027501 Replicon chromosome
Accession NZ_CP018671
Organism Klebsiella pneumoniae strain CAV1042

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag AGE78_RS07575 Protein ID WP_022615588.1
Coordinates 1026911..1027243 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag AGE78_RS07580 Protein ID WP_000288812.1
Coordinates 1027244..1027501 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGE78_RS07545 1022958..1023203 + 246 WP_023284006.1 signal transduction protein PmrD -
AGE78_RS07550 1023333..1024787 + 1455 WP_004148975.1 AMP nucleosidase -
AGE78_RS07560 1025153..1026589 - 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
AGE78_RS07575 1026911..1027243 - 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AGE78_RS07580 1027244..1027501 - 258 WP_000288812.1 antitoxin Antitoxin
AGE78_RS07585 1027836..1028042 - 207 WP_040153816.1 helix-turn-helix domain-containing protein -
AGE78_RS07590 1028039..1028500 - 462 WP_040153818.1 hypothetical protein -
AGE78_RS07600 1029420..1031168 - 1749 WP_074075367.1 hypothetical protein -
AGE78_RS30410 1031435..1031677 + 243 WP_040245131.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T70165 WP_022615588.1 NZ_CP018671:c1027243-1026911 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T70165 NZ_CP018671:c1027243-1026911 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT70165 WP_000288812.1 NZ_CP018671:c1027501-1027244 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT70165 NZ_CP018671:c1027501-1027244 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References