Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 176222..176748 Replicon plasmid pCAV1042-183
Accession NZ_CP018670
Organism Klebsiella pneumoniae strain CAV1042

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AGE78_RS02015 Protein ID WP_000323025.1
Coordinates 176222..176509 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AGE78_RS02020 Protein ID WP_000534858.1
Coordinates 176509..176748 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGE78_RS01965 171817..172047 - 231 WP_020805755.1 hypothetical protein -
AGE78_RS01970 172135..172482 - 348 WP_020316654.1 hypothetical protein -
AGE78_RS01975 172548..172928 - 381 WP_015632488.1 hypothetical protein -
AGE78_RS01985 173445..173795 - 351 WP_020277938.1 hypothetical protein -
AGE78_RS01990 173792..174064 - 273 WP_023317525.1 hypothetical protein -
AGE78_RS02000 174254..174739 + 486 WP_020277937.1 hypothetical protein -
AGE78_RS02005 174900..175061 - 162 WP_032426744.1 type I toxin-antitoxin system Hok family toxin -
AGE78_RS02010 175121..176155 + 1035 Protein_199 IS481 family transposase -
AGE78_RS02015 176222..176509 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AGE78_RS02020 176509..176748 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AGE78_RS02025 177011..177934 - 924 WP_020277922.1 cation diffusion facilitator family transporter -
AGE78_RS02030 178134..178706 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
AGE78_RS02040 179182..180420 - 1239 WP_003030308.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..183432 183432


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T70155 WP_000323025.1 NZ_CP018670:c176509-176222 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T70155 NZ_CP018670:c176509-176222 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT70155 WP_000534858.1 NZ_CP018670:c176748-176509 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT70155 NZ_CP018670:c176748-176509 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References