Detailed information of TA system TA070147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 29371..29896
• Replicon: plasmid pSE93-0639
• Accession: NZ_CP018660
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain 93-0639

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: BTN75_RS24125
• Protein ID: WP_001159863.1
• Coordinates: 29591..29896 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: BTN75_RS24120
• Protein ID: WP_000813641.1
• Coordinates: 29371..29589 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTN75_RS24090	24985..25371	+	387	WP_000751876.1	YgiW/YdeI family stress tolerance OB fold protein	-
BTN75_RS24095	25424..25585	+	162	WP_015059604.1	hypothetical protein	-
BTN75_RS24100	25912..26901	-	990	WP_000461382.1	RepB family plasmid replication initiator protein	-
BTN75_RS24105	27395..27690	-	296	Protein_39	cytoplasmic protein	-
BTN75_RS24565	27702..28130	+	429	Protein_40	hypothetical protein	-
BTN75_RS24115	28174..28695	-	522	WP_077681952.1	hypothetical protein	-
BTN75_RS24120	29371..29589	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
BTN75_RS24125	29591..29896	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
BTN75_RS24130	29898..30188	+	291	WP_001266176.1	hypothetical protein	-
BTN75_RS24135	30185..30706	+	522	WP_000198608.1	hypothetical protein	-
BTN75_RS24140	30741..31523	+	783	WP_000082169.1	site-specific integrase	-
BTN75_RS24145	31532..32245	+	714	WP_000545756.1	EAL domain-containing protein	-
BTN75_RS24150	32249..32757	+	509	Protein_48	CaiF/GrlA family transcriptional regulator	-
BTN75_RS24155	32751..33235	+	485	Protein_49	hypothetical protein	-
BTN75_RS24440	33512..33799	-	288	WP_071530243.1	hypothetical protein	-
BTN75_RS24160	33955..34515	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..59370	59370

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T70147 WP_001159863.1 NZ_CP018660:29591-29896 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T70147 NZ_CP018660:29591-29896 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT70147 WP_000813641.1 NZ_CP018660:29371-29589 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT70147 NZ_CP018660:29371-29589 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6